一种用于细胞内血红素的基因编码荧光共振能量转移传感器。

A Genetically Encoded FRET Sensor for Intracellular Heme.

作者信息

Song Yanqun, Yang Maiyun, Wegner Seraphine V, Zhao Jingyi, Zhu Rongfeng, Wu Yun, He Chuan, Chen Peng R

机构信息

†Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

‡Department of Chemistry and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, United States.

出版信息

ACS Chem Biol. 2015 Jul 17;10(7):1610-5. doi: 10.1021/cb5009734. Epub 2015 Apr 17.

DOI:10.1021/cb5009734

PMID:25860383

Abstract

Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.

摘要

血红素在各种细胞过程以及生物系统中的铁稳态中发挥着关键作用。在此，我们报道了一种通过使用一对细菌血红素转移伴侣作为传感组件来进行选择性血红素成像的基因编码荧光共振能量转移（FRET）传感器。这种血红素特异性探针能够对活细胞内的血红素分布进行时空可视化。