[三叉神经痛大鼠模型中痛阈及胶质细胞源性神经营养因子的变化]
[Changes in pain threshold and glial cell line-derived neurotrophic factor in rat model of trigeminal neuralgia].
作者信息
Sijia Qin, Xiaohong Zhang, Haiwei Jin, Lu Gao, Fu Wang
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Feb;33(1):16-20. doi: 10.7518/hxkq.2015.01.004.
OBJECTIVE
This research aims to study the changes in pain threshold and glial cell line-derived neurotrophic factor (GDNF) in a Sprague Dawley (SD) rat model oftrigeminal neuralgia.
METHODS
A total of 36 male SD rats were randomly divided into three groups: operative, sham-operative, and control. In the operative group, a chronic constriction injury (CCI) was caused by placing loose chromic gut ligatures around the right infraorbital nerve (ION). In the sham-operative group, the right ION was subjected to the same procedure, but without ligation. In the control group, the right ION was not subjected to any treatment. The pain thresholds of the three groups were recorded at different times after the operation. The GDNF expression in each group was analyzed via immunohistochemical staining.
RESULTS
An allodynia to mechanical stimulation in the region of the ligated ION was observed starting on the 2nd week after operation. Pain thresholds started to increase gradually from the 6th week and returned to the original level at the 10th to 12th week after operation. Cells that expressed the GDNF markedly increased in number in the operative group with changes observed at different times.
CONCLUSION
We use chronic constriction injury to the infraorbital nerve (CCI-ION) to establish a trigeminal neuralgia-like animal model in SD rats. GDNF may play a role in regulating pain by promoting the restoration and regeneration of nerve fibers.
目的
本研究旨在探讨三叉神经痛的Sprague Dawley（SD）大鼠模型中痛阈和胶质细胞源性神经营养因子（GDNF）的变化。
方法
将36只雄性SD大鼠随机分为三组：手术组、假手术组和对照组。手术组通过在右侧眶下神经（ION）周围放置松弛的铬肠线结扎造成慢性压迫性损伤（CCI）。假手术组对右侧ION进行相同操作，但不结扎。对照组右侧ION不进行任何处理。术后不同时间记录三组的痛阈。通过免疫组织化学染色分析每组中GDNF的表达。
结果
术后第2周开始观察到结扎ION区域对机械刺激的异常性疼痛。痛阈从术后第6周开始逐渐升高，并在术后第10至12周恢复到原始水平。手术组中表达GDNF的细胞数量明显增加，且在不同时间观察到变化。
结论
我们采用眶下神经慢性压迫性损伤（CCI-ION）在SD大鼠中建立三叉神经痛样动物模型。GDNF可能通过促进神经纤维的修复和再生在调节疼痛中发挥作用。
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