马来巴醇A以PPARγ依赖的方式调节实验性中风中微胶质细胞的M1/M2极化。
Malibatol A regulates microglia M1/M2 polarization in experimental stroke in a PPARγ-dependent manner.
作者信息
Pan Jie, Jin Jia-li, Ge Hui-ming, Yin Kai-lin, Chen Xiang, Han Li-juan, Chen Yan, Qian Lai, Li Xiao-xi, Xu Yun
机构信息
Department of Neurology, Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 Zhongshan Road, Nanjing, 210008, China.
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu, 210093, China.
出版信息
J Neuroinflammation. 2015 Mar 14;12:51. doi: 10.1186/s12974-015-0270-3.
BACKGROUND
Activation of microglia plays a crucial role in immune and inflammatory processes after ischemic stroke. Microglia is reported with two opposing activated phenotypes, namely, classic phenotype (M1) and the alternative phenotype (M2). Inhibiting M1 while stimulating M2 has been suggested as a potential therapeutic approach in the treatment of stroke.
FINDINGS
In this study, we indicated that a novel natural anti-oxidant extracted from the Chinese plant Hopea hainanensis, malibatol A (MA), decreased the infarct size and alleviated the brain injury after mice middle cerebral artery occlusion (MCAO). MA inhibited expression inflammatory cytokines in not only MCAO mice but also lipopolysaccharide (LPS)-stimulated microglia. Moreover, treatment of MA decreased M1 markers (CD16, CD32, and CD86) and increased M2 markers (CD206, YM-1) while promoting the activation of nuclear receptor PPARγ.
CONCLUSIONS
MA has anti-inflammatory effects in MCAO mice in a PPARγ-dependent manner, making it a potential candidate for stroke treatment.
背景
小胶质细胞的激活在缺血性中风后的免疫和炎症过程中起关键作用。据报道，小胶质细胞有两种相反的激活表型，即经典表型（M1）和替代表型（M2）。抑制M1同时刺激M2已被认为是治疗中风的一种潜在治疗方法。
研究结果
在本研究中，我们表明从中国植物海南坡垒中提取的一种新型天然抗氧化剂——海南坡垒醇A（MA），可减小小鼠大脑中动脉闭塞（MCAO）后的梗死面积并减轻脑损伤。MA不仅抑制MCAO小鼠中的炎性细胞因子表达，还抑制脂多糖（LPS）刺激的小胶质细胞中的炎性细胞因子表达。此外，MA处理降低了M1标志物（CD16、CD32和CD86），增加了M2标志物（CD206、YM-1），同时促进核受体PPARγ的激活。
结论
MA以PPARγ依赖的方式在MCAO小鼠中具有抗炎作用，使其成为中风治疗的潜在候选药物。
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