Liu Zhirui, Chen Shuanggang, Zhou You, Xie Cuihong, Zhu Bifeng, Zhu Huming, Liu Shupeng, Wang Wei, Chen Hongzhuan, Ji Yonghua
1] Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Nanchen Road 333, Shanghai 200444, P.R. China [2] Department of Pharmacology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, South Chongqing Road 280, Shanghai 200025, P.R. China.
Laboratory of Neuropharmacology and Neurotoxicology, Shanghai University, Nanchen Road 333, Shanghai 200444, P.R. China.
Sci Rep. 2015 Apr 23;5:9454. doi: 10.1038/srep09454.
Wasp stings have been arising to be a severe public health problem in China in recent years. However, molecular information about lethal or toxic factors in wasp venom is extremely lacking. In this study, we used two pyrosequencing platforms to analyze the transcriptome of Vespa velutina, the most common wasp species native in China. Besides the substantial amount of transcripts encoding for allergens usually regarded as the major lethal factor of wasp sting, a greater abundance of hemostasis-impairing toxins and neurotoxins in the venom of V. velutina were identified, implying that toxic reactions and allergic effects are envenoming strategy for the dangerous outcomes. The pattern of differentially expressed genes before and after venom extraction clearly indicates that the manifestation of V. velutina stings depends on subtle regulations in the metabolic pathway required for toxin recruitment. This comparative analysis offers timely clues for developing clinical treatments for wasp envenoming in China and around the world.
近年来，黄蜂蜇伤在中国已逐渐成为一个严重的公共卫生问题。然而，关于黄蜂毒液中致死或有毒因子的分子信息极其匮乏。在本研究中，我们使用两个焦磷酸测序平台分析了中国本土最常见的黄蜂物种——黄脚胡蜂的转录组。除了大量编码通常被视为黄蜂蜇伤主要致死因子的过敏原的转录本外，还鉴定出黄脚胡蜂毒液中大量的促凝血毒素和神经毒素，这意味着毒性反应和过敏效应是导致危险后果的毒液策略。毒液提取前后差异表达基因的模式清楚地表明，黄脚胡蜂蜇伤的表现取决于毒素募集所需代谢途径中的微妙调控。这种比较分析为中国乃至全球开发黄蜂蜇伤的临床治疗方法提供了及时的线索。
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