[脂肪酸合酶和脂肪细胞脂肪酸结合蛋白在人浸润性导管乳腺癌中的表达及其与临床病理特征的关系]

[Expression of fatty acid synthase and adipocyte fatty acid-binding protein and the relationship with the clinicopathological characteristics in human infiltrating ductal breast cancer].

作者信息

Zhou Lan, Zhao Yu-hua, Wang Xiao-dong, Jiang Su-fang, Li Hua

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 Mar;46(2):228-33.

Abstract

OBJECTIVE

To study the expression of fatty acid synthase (FASN) and adipocyte fatty acid-binding protein (A-FABP) in human infiltrating ductal breast cancer (IDC) tissues and hunman breast cancer cells and the relationship with the clinicopathogical characteristics. To further explore the relationship between FASN and A-FABP, and the relevance of the invasion in cancer cell.

METHODS

The expression of FASN and A-FABP was detected in 58 cases of human infiltrating ductal breast cancer and 12 cases of human normal breast tissues by immunohistochemistry technique, calculated positive expression percentage according to the number of positive cells percentage and the staining degree of positive sediment. The cell wound-healing assay was applied to detect the invasion of SKBR3 and MCF-7 cells. Western blot was used to detect the expression of FASN and A-FABP in MCF-7 and SKBR3 cells.

RESULTS

The positive rates of FASN and A-FABP were 8.3% (1/12) and 16.7% (1/6) respectively in 12 cases of normal breast tissues by immunohistochemistry. In 58 cases of IDC tissues, the positive rates of FASN and A-FABP were 72.4% (42/58) and 79.3% (46/58) respectively. The differences of the positive rates of FASN and A-FABP in normal breast and IDC tissues were statistically significant (P<0.01, P<O=0.05). The expression of FASN and A-FABP was up-regulated in the lymph node metastasis positive group and the larger size group (tumor diameter>2 cm) when compared with lymph node metastasis negative group or the diameter < or =2 cm group, the differences were statistically significant (P<0.01). In IDC group, the expression of FASN correlated with A-FABP (r=0.797, P<0.001), The migration rate of SKBR3 was significantly higher than MCF-7 cell at 12, 24 h (P<0.05), FASN expression in SKBR3 was higher than that in MCF-7.

CONCLUSION

FASN and A-FABP might associated with the lymph node metastasis and tumor size, and there was correlation between FASN and A-FABP in human IDC tissues. FASN may associated with the invasion and metastasis in breast cancer cells.

摘要

目的

研究脂肪酸合酶（FASN）和脂肪细胞脂肪酸结合蛋白（A-FABP）在人浸润性导管乳腺癌（IDC）组织及人乳腺癌细胞中的表达情况及其与临床病理特征的关系。进一步探讨FASN与A-FABP之间的关系以及与癌细胞侵袭的相关性。

方法

采用免疫组织化学技术检测58例人浸润性导管乳腺癌组织和12例人正常乳腺组织中FASN和A-FABP的表达，根据阳性细胞百分比和阳性沉淀物染色程度计算阳性表达率。应用细胞划痕实验检测SKBR3和MCF-7细胞的侵袭能力。采用蛋白质免疫印迹法检测MCF-7和SKBR3细胞中FASN和A-FABP的表达。

结果

免疫组织化学检测显示，12例正常乳腺组织中FASN和A-FABP的阳性率分别为8.3%（1/12）和16.7%（1/6）。在58例IDC组织中，FASN和A-FABP的阳性率分别为72.4%（42/58）和79.3%（46/58）。正常乳腺组织与IDC组织中FASN和A-FABP阳性率的差异具有统计学意义（P<0.01，P<0.05）。与淋巴结转移阴性组或肿瘤直径≤2 cm组相比，淋巴结转移阳性组和肿瘤较大组（肿瘤直径>２cm）中FASN和A-FABP的表达上调，差异具有统计学意义（P<0.01）。在IDC组中，FASN的表达与A-FABP相关（r=0.797，P<0.001），在12、24 h时SKBR3细胞的迁移率显著高于MCF-7细胞（P<0.05），SKBR3细胞中FASN的表达高于MCF-7细胞。