对在朝鲜古墓粪化石中发现的蛔虫卵进行古代线粒体DNA分析。
Ancient mitochondrial DNA analyses of ascaris eggs discovered in coprolites from joseon tomb.
作者信息
Oh Chang Seok, Seo Min, Hong Jong Ha, Chai Jong-Yil, Oh Seung Whan, Park Jun Bum, Shin Dong Hoon
机构信息
Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, Seoul 110-799, Korea.
Department of Parasitology and Research Center for Mummy, Dankook University, Cheonan 330-715, Korea.
出版信息
Korean J Parasitol. 2015 Apr;53(2):237-42. doi: 10.3347/kjp.2015.53.2.237. Epub 2015 Apr 22.
Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.
从蛔虫中提取古代DNA（aDNA）对于了解该寄生虫物种的系统发育谱系非常重要。当用细胞色素b（cyt b）和18S小亚基核糖体RNA（18S rRNA）基因的引物对从一个鉴定出蛔虫卵的朝鲜古墓（SN2-19-1）粪化石中获得的aDNA进行扩增时，结果显示出蛔虫特异性扩增条带。通过对扩增DNA的克隆、测序和分析，我们获得了对于理解朝鲜古代人群中普遍存在的蛔虫遗传谱系有价值的信息。
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