围产期营养不良通过奖赏刺激动机，并增强成年小鼠腹侧纹状体中drd（1a）受体的表达。

Perinatal malnutrition stimulates motivation through reward and enhances drd(1a) receptor expression in the ventral striatum of adult mice.

作者信息

de Melo Martimiano Paula Honório, da Silva Gilvanildo Roberto, Coimbra Vanessa Fernandes da Silva Almeida, Matos Rhowena Jane Barbosa, de Souza Bruno Fernando Pereira, da Silva Amanda Alves Marcelino, de Melo Diogo D'Paula Cunha Brasileiro, de Souza Sandra Lopes, de Freitas Manuela Figueiroa Lyra

机构信息

PPG - Neuropsychiatry and Behaviour Sciences, Federal University of Pernambuco, Prof° Moraes Rego Avenue, Recife, PE, Brazil.

PPG - Pathology, Federal University of Pernambuco, Prof° Moraes Rego Avenue, Recife, PE, Brazil.

出版信息

Pharmacol Biochem Behav. 2015 Jul;134:106-14. doi: 10.1016/j.pbb.2015.04.008. Epub 2015 Apr 28.

DOI:10.1016/j.pbb.2015.04.008

PMID:25933794

Abstract

AIM

The aim of this study was to analyze the effects of protein perinatal malnutrition on the function of dopamine DRD1 and DRD2 receptors in regards to motivation and food consumption in adult mice. The study also analyzed the effect of protein perinatal malnutrition on the gene expression of these receptors in the ventral striatum.

METHODS

Wistar lineage mice were divided into two groups according to maternal diet: control (17% casein), n=30 and low protein (8% casein), n=30. Between 30 and 120days of life, the following factors were measured: body weight; the effect of dopamine D1 and D2 agonists on the ingestion of palatable food; the motivational aspect under the action of the D1 (SKF 38393) and D2 Quinpirole dopaminergic agonists; and the gene expression of DRD1 and DRD2 receptors in the ventral striatum.

RESULTS

The body weights of the malnourished animals remained significantly lower than those of the control group from 30 to 120days of life. Malnourished animals ingested a greater quantity of palatable food. There was a decrease in palatable diet consumption in both the control and malnourished groups after the application of D1 and D2 agonists; however, the anorexic effect of the D1 agonist was understated in malnourished animals. Perinatal malnutrition increases the motivational behavior of the animal when food reward is used. There was an increase in gene expression of the DRD1a receptor in the ventral striatum of malnourished animals, and there were no significant changes concerning the DRD2 receptor.

CONCLUSIONS

Perinatal protein malnutrition stimulates hedonic control of eating behavior by promoting increased intake of palatable foods, possibly due to increased expression of dopamine receptor DRD1a in the ventral striatum.

摘要

目的

本研究旨在分析围产期蛋白质营养不良对成年小鼠动机及食物消耗方面多巴胺DRD1和DRD2受体功能的影响。该研究还分析了围产期蛋白质营养不良对腹侧纹状体中这些受体基因表达的影响。

方法

根据母鼠饮食将Wistar品系小鼠分为两组：对照组（17%酪蛋白），n = 30；低蛋白组（8%酪蛋白），n = 30。在出生后30至120天期间，测量以下因素：体重；多巴胺D1和D2激动剂对美味食物摄取的影响；D1（SKF 38393）和D2喹吡罗多巴胺能激动剂作用下的动机方面；以及腹侧纹状体中DRD1和DRD2受体的基因表达。

结果

在出生后30至120天期间，营养不良动物的体重显著低于对照组。营养不良动物摄取了更多的美味食物。应用D1和D2激动剂后，对照组和营养不良组的美味饮食消耗均减少；然而，D1激动剂的厌食作用在营养不良动物中被低估。当使用食物奖励时，围产期营养不良会增加动物的动机行为。营养不良动物腹侧纹状体中DRD1a受体的基因表达增加，而DRD2受体无显著变化。