培养条件对炭角菌属代谢产物产生的影响
Effect of Culture Conditions on Metabolite Production of Xylaria sp.
作者信息
Zhang Hongqi, Deng Zhangshuang, Guo Zhiyong, Peng Yan, Huang Nianyu, He Haibo, Tu Xuan, Zou Kun
机构信息
Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China.
The First College of Clinical Medical Science, China Three Gorges University, Yichang 443002, China.
出版信息
Molecules. 2015 Apr 30;20(5):7940-50. doi: 10.3390/molecules20057940.
Seeking a strategy for triggering the cryptic natural product biosynthesis to yield novel compounds in the plant-associated fungus Xylaria sp., the effect of culture conditions on metabolite production was investigated. A shift in the production of five known cytochalasin-type analogues 1-5 to six new α-pyrone derivatives, xylapyrones A-F (compounds 6-11), from a solid to a liquid medium was observed. These compounds were identified by analysis of 1D and 2D NMR and HRMS data. Compounds 1-3 showed moderate cytotoxicity against HepG2 and Caski cancer cell lines with IC50 values ranging from 25 to 63 μM and compounds 4-11 were found to be inactive, with IC50 values>100 μM.
为了寻找一种触发神秘天然产物生物合成以在与植物相关的真菌木层孔菌属中产生新化合物的策略，研究了培养条件对代谢产物产生的影响。观察到从固体培养基转变为液体培养基时，五种已知的细胞松弛素类类似物1-5的产量发生了变化，产生了六种新的α-吡喃酮衍生物，即木层孔菌素A-F（化合物6-11）。通过对一维和二维核磁共振以及高分辨率质谱数据的分析鉴定了这些化合物。化合物1-3对HepG2和Caski癌细胞系表现出中等细胞毒性，IC50值范围为25至63 μM，而化合物4-11被发现无活性，IC50值>100 μM。
Zotero 插件