热带地区类圆线虫病的分子诊断：传统聚合酶链反应和实时聚合酶链反应与寄生虫学方法的比较
Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods.
作者信息
Paula Fabiana Martins de, Malta Fernanda de Mello, Marques Priscilla Duarte, Sitta Renata Barnabé, Pinho João Renato Rebello, Gryschek Ronaldo César Borges, Chieffi Pedro Paulo
机构信息
Instituto de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias.
出版信息
Mem Inst Oswaldo Cruz. 2015 Apr;110(2):272-4. doi: 10.1590/0074-02760140371. Epub 2015 Mar 6.
Abstract
This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.
摘要
本研究旨在评估传统聚合酶链反应（cPCR）和实时定量聚合酶链反应（qPCR）在热带地区粪便样本诊断人体类圆线虫病中的应用。从个体采集粪便样本，并确定其粪类圆线虫呈阳性（第一组）、粪类圆线虫呈阴性（第二组）以及其他肠道寄生虫种类呈阳性（第三组）。通过cPCR在第一组样本的76.7%中检测到粪类圆线虫特异性DNA，通过qPCR在第一组样本的90%中检测到该DNA。结果表明，分子方法可作为热带地区人体粪便样本中检测粪类圆线虫的替代工具。
相似文献
1
Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods.热带地区类圆线虫病的分子诊断：传统聚合酶链反应和实时聚合酶链反应与寄生虫学方法的比较
Mem Inst Oswaldo Cruz. 2015 Apr;110(2):272-4. doi: 10.1590/0074-02760140371. Epub 2015 Mar 6.
2
Molecular diagnosis of Strongyloides stercoralis among transplant candidates.移植候选者中粪类圆线虫的分子诊断
Transpl Infect Dis. 2018 Aug;20(4):e12909. doi: 10.1111/tid.12909. Epub 2018 May 14.
3
Conventional PCR for molecular diagnosis of human strongyloidiasis.常规 PCR 用于人体旋毛虫病的分子诊断。
Parasitology. 2014 Apr;141(5):716-21. doi: 10.1017/S0031182013002035. Epub 2014 Jan 28.
4
Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples.巢式聚合酶链反应和实时聚合酶链反应与寄生虫学方法在检测人粪便样本中粪类圆线虫的比较
Am J Trop Med Hyg. 2015 Dec;93(6):1285-91. doi: 10.4269/ajtmh.15-0309. Epub 2015 Sep 8.
5
Real-time PCR for detection of Strongyloides stercoralis in human stool samples from Côte d'Ivoire: diagnostic accuracy, inter-laboratory comparison and patterns of hookworm co-infection.用于检测来自科特迪瓦人粪便样本中粪类圆线虫的实时聚合酶链反应：诊断准确性、实验室间比较及钩虫合并感染模式
Acta Trop. 2015 Oct;150:210-7. doi: 10.1016/j.actatropica.2015.07.019. Epub 2015 Jul 26.
6
An improved DNA isolation technique for PCR detection of Strongyloides stercoralis in stool samples.一种用于粪便样本中粪类圆线虫PCR检测的改良DNA提取技术。
Acta Trop. 2013 May;126(2):110-4. doi: 10.1016/j.actatropica.2013.02.003. Epub 2013 Feb 12.
7
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis.分子生物学技术诊断粪类圆线虫感染的准确性：系统评价和荟萃分析。
PLoS Negl Trop Dis. 2018 Feb 9;12(2):e0006229. doi: 10.1371/journal.pntd.0006229. eCollection 2018 Feb.
8
Strong-LAMP: A LAMP Assay for Strongyloides spp. Detection in Stool and Urine Samples. Towards the Diagnosis of Human Strongyloidiasis Starting from a Rodent Model.强LAMP：一种用于检测粪便和尿液样本中类圆线虫属的环介导等温扩增检测法。从啮齿动物模型出发迈向人类类圆线虫病的诊断。
PLoS Negl Trop Dis. 2016 Jul 14;10(7):e0004836. doi: 10.1371/journal.pntd.0004836. eCollection 2016 Jul.
9
Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool.实时聚合酶链反应检测粪便中的粪类圆线虫。
Am J Trop Med Hyg. 2013 Jun;88(6):1048-51. doi: 10.4269/ajtmh.12-0437. Epub 2013 Apr 8.
10
A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of Strongyloides stercoralis infection.一项比较琼脂平板培养、间接免疫荧光法和实时聚合酶链反应诊断粪类圆线虫感染的回顾性研究。
Parasitology. 2017 May;144(6):812-816. doi: 10.1017/S0031182016002559. Epub 2017 Jan 11.
引用本文的文献
1
Frequency of strongyloidiasis and associated factors: Analysis of 13 years of laboratory results in a tertiary referral hospital in Honduras, 2010-2022.寄生虫感染的频率及相关因素分析：洪都拉斯一家三级转诊医院 2010-2022 年 13 年的实验室结果分析。
Biomedica. 2023 Dec 1;43(4):492-505. doi: 10.7705/biomedica.7086.
2
From past to present: opportunities and trends in the molecular detection and diagnosis of Strongyloides stercoralis.从过去到现在：粪类圆线虫分子检测和诊断的机会和趋势。
Parasit Vectors. 2023 Apr 11;16(1):123. doi: 10.1186/s13071-023-05763-8.
3
Strongyloides stercoralis hyperinfection presenting with shock and intermittent eosinophilia: A case report.粪类圆线虫重度感染致感染性休克并间息性嗜酸性粒细胞增多：一例报告。
Medicine (Baltimore). 2022 Sep 2;101(35):e30490. doi: 10.1097/MD.0000000000030490.
4
Development and Efficacy of Droplet Digital PCR for Detection of Strongyloides stercoralis in Stool.粪便中粪类圆线虫的液滴数字 PCR 检测的开发与功效。
Am J Trop Med Hyg. 2021 Oct 18;106(1):312-319. doi: 10.4269/ajtmh.21-0729.
5
Prevalence of strongyloidiasis in the general population of the world: a systematic review and meta-analysis.世界普通人群中类圆线虫病的流行情况：系统评价和荟萃分析。
Pathog Glob Health. 2021 Feb;115(1):7-20. doi: 10.1080/20477724.2020.1851922. Epub 2021 Jan 12.
6
Strongyloidiasis in Africa: Systematic Review and Meta-Analysis on Prevalence, Diagnostic Methods, and Study Settings.非洲的旋毛虫病：流行率、诊断方法和研究环境的系统评价和荟萃分析。
Biomed Res Int. 2020 Nov 15;2020:2868564. doi: 10.1155/2020/2868564. eCollection 2020.
7
Comparison of Loop-Mediated Isothermal Amplification and Real-Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices.环介导等温扩增法与实时 PCR 法检测不同标本基质中粪类圆线虫幼虫的比较。
J Clin Microbiol. 2019 Mar 28;57(4). doi: 10.1128/JCM.01173-18. Print 2019 Apr.
8
Diagnostic comparison of Baermann funnel, Koga agar plate culture and polymerase chain reaction for detection of human Strongyloides stercoralis infection in Maluku, Indonesia.印度尼西亚马鲁古省用于检测人体粪类圆线虫感染的贝尔曼漏斗法、古贺琼脂平板培养法和聚合酶链反应的诊断比较
Parasitol Res. 2018 Oct;117(10):3229-3235. doi: 10.1007/s00436-018-6021-5. Epub 2018 Aug 3.
9
Molecular and Immnune Diagnosis: Further Testing for Human Strongyloidiasis.分子与免疫诊断：人鞭虫病的进一步检测。
Mol Diagn Ther. 2018 Aug;22(4):485-491. doi: 10.1007/s40291-018-0340-1.
10
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis.分子生物学技术诊断粪类圆线虫感染的准确性：系统评价和荟萃分析。
PLoS Negl Trop Dis. 2018 Feb 9;12(2):e0006229. doi: 10.1371/journal.pntd.0006229. eCollection 2018 Feb.
本文引用的文献
1
Diagnosis, treatment and risk factors of Strongyloides stercoralis in schoolchildren in Cambodia.柬埔寨学童中 Strongyloides stercoralis 的诊断、治疗和危险因素。
PLoS Negl Trop Dis. 2013;7(2):e2035. doi: 10.1371/journal.pntd.0002035. Epub 2013 Feb 7.
2
Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia.评估实时荧光定量PCR用于检测柬埔寨无症状学童体内粪类圆线虫和钩虫的诊断价值。
Acta Trop. 2013 May;126(2):89-92. doi: 10.1016/j.actatropica.2012.12.012. Epub 2013 Jan 5.
3
Screening, prevention, and treatment for hyperinfection syndrome and disseminated infections caused by Strongyloides stercoralis.筛查、预防和治疗粪类圆线虫引起的超感染综合征和播散性感染。
Curr Opin Infect Dis. 2012 Aug;25(4):458-63. doi: 10.1097/QCO.0b013e3283551dbd.
4
Molecular Diagnosis of Strongyloides stercoralis Infection by PCR Detection of Specific DNA in Human Stool Samples.通过聚合酶链反应检测人类粪便样本中的特异性DNA对粪类圆线虫感染进行分子诊断。
Iran J Parasitol. 2011 Jun;6(2):23-30.
5
Efficacy of parasitological methods for the diagnosis of Strongyloides stercoralis and hookworm in faecal specimens.粪便标本中粪类圆线虫和钩虫寄生虫学方法诊断效果的研究。
Acta Trop. 2011 Dec;120(3):206-10. doi: 10.1016/j.actatropica.2011.08.010. Epub 2011 Aug 27.
6
Epidemiological aspects of strongyloidiasis in Brazil.巴西旋毛虫病的流行病学方面。
Parasitology. 2011 Sep;138(11):1331-40. doi: 10.1017/S003118201100120X. Epub 2011 Aug 3.
7
Prevalence of intestinal parasites in preschool children in the region of Uberlândia, State of Minas Gerais, Brazil.巴西米纳斯吉拉斯州乌贝兰迪亚地区学龄前儿童肠道寄生虫感染的流行情况。
Rev Soc Bras Med Trop. 2011 Mar-Apr;44(2):191-3. doi: 10.1590/s0037-86822011005000022. Epub 2011 Apr 29.
8
Molecular diagnostics of intestinal parasites in returning travellers.回国旅行者肠道寄生虫的分子诊断
Eur J Clin Microbiol Infect Dis. 2009 Sep;28(9):1045-53. doi: 10.1007/s10096-009-0745-1. Epub 2009 May 5.
9
Strongyloidiasis--the most neglected of the neglected tropical diseases?类圆线虫病——被忽视的热带病中最被忽视的疾病？
Trans R Soc Trop Med Hyg. 2009 Oct;103(10):967-72. doi: 10.1016/j.trstmh.2009.02.013. Epub 2009 Mar 27.
10
Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR.使用实时聚合酶链反应对粪便样本中粪类圆线虫进行分子诊断。
Trans R Soc Trop Med Hyg. 2009 Apr;103(4):342-6. doi: 10.1016/j.trstmh.2008.12.001. Epub 2009 Feb 4.
Zotero 插件