中华蜜蜂中一个新的1-半胱氨酸硫氧还蛋白过氧化物酶基因：AccTpx4的特性及其在氧化应激中的作用
A novel 1-Cys thioredoxin peroxidase gene in Apis cerana cerana: characterization of AccTpx4 and its role in oxidative stresses.
作者信息
Huaxia Yifeng, Wang Fang, Yan Yan, Liu Feng, Wang Hongfang, Guo Xingqi, Xu Baohua
机构信息
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, People's Republic of China.
出版信息
Cell Stress Chaperones. 2015 Jul;20(4):663-72. doi: 10.1007/s12192-015-0594-z. Epub 2015 May 15.
Thioredoxin peroxidase (Tpx), also named peroxiredoxin (Prx), is an important peroxidase that can protect organisms against stressful environments. AccTpx4, a 1-Cys thioredoxin peroxidase gene from the Chinese honey bee Apis cerana cerana, was cloned and characterized. The AccTpx4 gene encodes a protein that is predicted to contain the conserved PVCTTE motif from 1-Cys peroxiredoxin. Quantitative real-time PCR (Q-PCR) and Western blotting revealed that AccTpx4 was induced by various oxidative stresses, such as cold, heat, insecticides, H(2)O(2), and HgCl(2). The in vivo peroxidase activity assay showed that recombinant AccTpx4 protein could efficiently degrade H(2)O(2) in the presence of DL-dithiothreitol (DTT). In addition, disc fusion assays revealed that AccTpx4 could function to protect cells against oxidative stresses. These results indicate that AccTpx4 plays an important role in oxidative stress responses and may contribute to the conservation of honeybees.
硫氧还蛋白过氧化物酶（Tpx），也称为过氧化物氧还蛋白（Prx），是一种重要的过氧化物酶，可保护生物体免受应激环境的影响。克隆并鉴定了中华蜜蜂（Apis cerana cerana）的1-半胱氨酸硫氧还蛋白过氧化物酶基因AccTpx4。AccTpx4基因编码一种蛋白质，预计该蛋白质含有来自1-半胱氨酸过氧化物氧还蛋白的保守PVCTTE基序。实时定量PCR（Q-PCR）和蛋白质免疫印迹分析表明，AccTpx4受到各种氧化应激的诱导，如寒冷、高温、杀虫剂、H₂O₂和HgCl₂。体内过氧化物酶活性测定表明，重组AccTpx4蛋白在二硫苏糖醇（DTT）存在下可有效降解H₂O₂。此外，圆盘融合试验表明，AccTpx4可发挥作用保护细胞免受氧化应激。这些结果表明，AccTpx4在氧化应激反应中起重要作用，可能有助于蜜蜂的生存。
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