Bambauer R, Jutzler G A, Schmidt R, Weber U, el-Saadi R, Oest A, Jung W K
Department of Nephrology, University of Saarland, Homburg/Saar, FRG.
ASAIO Trans. 1989 Jul-Sep;35(3):516-9. doi: 10.1097/00002480-198907000-00111.
Since the quality of water in dialysis fluid varies considerably, and, in view of the fact that endotoxin or active derivatives can cause acute side effects in patients, the dialysis fluid must be sterile. The predialyzer fluid in 20 hemodialysis patients was investigated. Bacterial loading was between 5/ml and 5,000/ml, and the endotoxin concentration was high and extremely variable. After ultrafiltration of the dialysis fluid by a polyamide hollow fiber membrane, all samples were free of bacteria, and the concentration of endotoxin was lower than the detectable limit. With this procedure we can obtain sterile dialysis fluid, which is endotoxin free.
由于透析液的水质差异很大，而且鉴于内毒素或其活性衍生物会在患者身上引发急性副作用，所以透析液必须无菌。对20名血液透析患者的透析前液进行了研究。细菌载量在每毫升5个至5000个之间，内毒素浓度很高且变化极大。通过聚酰胺中空纤维膜对透析液进行超滤后，所有样本均无菌，内毒素浓度低于可检测限。通过此程序，我们可以获得无内毒素的无菌透析液。
