Bortolotti Murielle, Ventura Gabrielle, Jegatheesan Prasanthi, Choisy Caroline, Cynober Luc, De Bandt Jean-Pascal
EA4466 - PRETRAM, Faculty of Pharmacy, Paris Descartes University, Sorbonne Paris Cité, Paris, France.
EA4466 - PRETRAM, Faculty of Pharmacy, Paris Descartes University, Sorbonne Paris Cité, Paris, France; Clinical Chemistry Department, Hôpitaux Universitaires Paris Centre, AP-HP, Paris, France.
Clin Nutr. 2016 Jun;35(3):669-78. doi: 10.1016/j.clnu.2015.04.015. Epub 2015 Apr 29.
Optimization of the refeeding strategy for the management of malnutrition in early life may enable to improve the quality of catch-up growth. While some data suggest better assimilation of peptides rather than whole proteins the evidence are scarce.
To compare the nutritional properties of peptides, partially hydrolyzed proteins or whole proteins in food-deprived/refed young rats.
Male SD rats (n = 109, 60-70 g) were food-deprived for 48 h and refed for 2-13 days. According to the set of experiments, refeeding was performed at 90% or 100% basal spontaneous intake or ad libitum with either peptide-, partially hydrolyzed protein- or whole protein-containing pediatric enteral nutrition formulas. Body weight, caloric intake and nitrogen balance were measured daily, intestinal trophicity was measured after two-day refeeding, and body composition was determined at the end of the refeeding period.
A 2-day food deprivation in young rats led to significant body and organ weight losses, alterations of gut morphology, and decreased plasma citrulline, a marker of gut function. A cautious 2-day renutrition at 90% pre-deprivation level did not restore nutritional status whatever the form of nitrogen supply. Ad libitum feeding was shown to be feasible with improved nitrogen efficiency. After 13 days, compared to chow diet, body weight gain was the lowest with peptide- and whole protein-containing diets, and significantly improved with partially hydrolyzed proteins with limited improvement in body lean mass (+8%, NS). Additional experiments indicated that in this model it will be necessary to significantly increase nitrogen supply in order to restore initial body weight and lean body mass.
Our results show benefits of ad libitum refeeding on catch-up growth. Our data suggest that partially hydrolyzed proteins may be beneficial in terms of body weight gain but that probably their effectiveness may be improved with higher nitrogen supply.
优化早期营养不良管理中的再喂养策略可能有助于提高追赶生长的质量。虽然一些数据表明肽比完整蛋白质具有更好的同化作用，但相关证据很少。
比较食物剥夺/再喂养的幼鼠对肽、部分水解蛋白或完整蛋白的营养特性。
雄性SD大鼠（n = 109，60 - 70 g）食物剥夺48小时，再喂养2 - 13天。根据实验设置，以90%或100%的基础自发摄入量或自由采食的方式，用含肽、部分水解蛋白或完整蛋白的儿科肠内营养配方进行再喂养。每天测量体重、热量摄入和氮平衡，再喂养两天后测量肠道营养状况，再喂养期结束时测定身体组成。
幼鼠两天的食物剥夺导致体重和器官重量显著减轻、肠道形态改变以及血浆瓜氨酸（肠道功能标志物）降低。以剥夺前水平的90%进行谨慎的两天再营养，无论氮供应形式如何，均不能恢复营养状况。自由采食被证明是可行的，且氮效率有所提高。13天后，与正常饮食相比，含肽和完整蛋白的饮食组体重增加最少，部分水解蛋白组体重显著增加，身体瘦体重仅有有限改善（+8%，无显著性差异）。额外实验表明，在该模型中，有必要显著增加氮供应以恢复初始体重和瘦体重。
我们的结果显示自由采食再喂养对追赶生长有益。我们的数据表明，部分水解蛋白在体重增加方面可能有益，但可能通过增加氮供应来提高其有效性。
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