通过飞行时间二次离子质谱对微通道中活生物膜进行二维和三维动态成像。
Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry.
作者信息
Hua Xin, Marshall Matthew J, Xiong Yijia, Ma Xiang, Zhou Yufan, Tucker Abigail E, Zhu Zihua, Liu Songqin, Yu Xiao-Ying
机构信息
Biological Sciences Division, Pacific Northwest National Laboratory , Richland, Washington 99354, USA.
College of Osteopathic Medicine of the Pacific-Northwest, Western University of Health Sciences , Lebanon, Oregon 97355, USA.
出版信息
Biomicrofluidics. 2015 May 5;9(3):031101. doi: 10.1063/1.4919807. eCollection 2015 May.
Abstract
A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface), was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. Two-dimensional (2D) images were reconstructed to report the first three-dimensional images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.
摘要
一种与真空兼容的微流控反应器SALVI（液体真空界面分析系统）被用于使用飞行时间二次离子质谱（ToF-SIMS）对活生物膜进行原位化学成像。通过逐层溅射材料进行深度剖析，实现了活生物膜的空间化学图谱绘制。重建二维（2D）图像以报告水合生物膜的首张三维图像，阐明其空间和化学异质性。对微通道中不同位置的生物膜进行了二维图像主成分分析。我们的方法通过动态液体ToF-SIMS直接可视化了活生物膜内的空间和化学异质性。
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