鼠尾草（药用鼠尾草）和茴香（小茴香）对冷冻保存的公猪附睾精液质量的改善研究。
Sage (Salvia officinalis) and fennel (Foeniculum vulgare) improve cryopreserved boar epididymal semen quality study.
作者信息
Monton A, Gil L, Malo C, Olaciregui M, Gonzalez N, de Blas I
机构信息
Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
Department of Animal Pathology, Infectious Diseases Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
出版信息
Cryo Letters. 2015 Mar-Apr;36(2):83-90.
The aim of this study was to evaluate the effect of fennel and sage extracts and the influence of the egg yolk source (fresh or pasteurized) on the success of freezing boar epididymal spermatozoa. In experiment 1, epididymal sperm was recovered by flushing and cryopreserved in a lactose-egg yolk solution supplemented with various concentrations (10, 5 and 2.5 g/L) of sage or fennel. Sperm quality was evaluated (motility, viability, HOST and acrosome integrity) at 0 h and 2 h after thawing. Fennel 10 g/L and sage 5 g/L and control (no extracts) were selected for experiment 2 which also compared fresh or pasteurized egg yolk in the freezing extender and measured DNA integrity of the frozen sperm. Results showed that the interaction between fennel and sage antioxidants with fresh egg yolk significantly improved post thaw sperm quality and protected boar epididymal spermatozoa from cryopreservation damage as a result of oxidative stress.
本研究的目的是评估茴香和鼠尾草提取物的作用以及蛋黄来源（新鲜或巴氏杀菌）对冷冻公猪附睾精子成功率的影响。在实验1中，通过冲洗回收附睾精子，并在补充了不同浓度（10、5和2.5 g/L）鼠尾草或茴香的乳糖-蛋黄溶液中进行冷冻保存。在解冻后0小时和2小时评估精子质量（活力、生存力、HOST和顶体完整性）。选择10 g/L茴香、5 g/L鼠尾草和对照组（无提取物）进行实验2，该实验还比较了冷冻稀释液中新鲜或巴氏杀菌蛋黄，并检测了冷冻精子的DNA完整性。结果表明，茴香和鼠尾草抗氧化剂与新鲜蛋黄之间的相互作用显著改善了解冻后精子质量，并保护公猪附睾精子免受冷冻保存过程中氧化应激造成的损伤。
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