新型半乳糖-N-二糖衍生物及其唾液酸化形式的高效化学酶法合成
Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms.
作者信息
Li Lei, Liu Yonghui, Li Tiehai, Wang Wenjun, Yu Zaikuan, Ma Cheng, Qu Jingyao, Zhao Wei, Chen Xi, Wang Peng G
机构信息
Department of Chemistry and Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30303, USA.
出版信息
Chem Commun (Camb). 2015 Jun 28;51(51):10310-3. doi: 10.1039/c5cc03746h.
Abstract
Galacto-N-biose (GNB) derivatives were efficiently synthesized from galactose derivatives via a one-pot two-enzyme system containing two promiscuous enzymes from Bifidobacterium infantis: a galactokinase (BiGalK) and a d-galactosyl-β1-3-N-acetyl-d-hexosamine phosphorylase (BiGalHexNAcP). Mono-sialyl and di-sialyl galacto-N-biose derivatives were then prepared using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α2-3-sialyltransferase or an α2-6-sialyltransferase.
摘要
通过包含来自婴儿双歧杆菌的两种混杂酶的一锅双酶系统，由半乳糖衍生物高效合成了半乳糖-N-双糖（GNB）衍生物：一种半乳糖激酶（BiGalK）和一种D-半乳糖基-β1-3-N-乙酰-D-己糖胺磷酸化酶（BiGalHexNAcP）。然后使用包含CMP-唾液酸合成酶和α2-3-唾液酸转移酶或α2-6-唾液酸转移酶的一锅双酶系统制备了单唾液酸和双唾液酸半乳糖-N-双糖衍生物。
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