抗 MDM2 自身抗体：系统性红斑狼疮的一种潜在血清学标志物。

Autoantibody to MDM2: A Potential Serological Marker of Systemic Lupus Erythematosus.

机构信息

Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital, Xiamen University, No. 55, Zhenhai Road, Xiamen 361003, China ; Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.

Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX 79968, USA.

出版信息

J Immunol Res. 2015;2015:963568. doi: 10.1155/2015/963568. Epub 2015 May 18.

DOI:10.1155/2015/963568

PMID:26090506

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4451996/

Abstract

Introduction. Systemic lupus erythematosus (SLE) is one of the systemic autoimmune diseases characterized by the polyclonal autoantibody production. The human homologue of the mouse double minute 2 (MDM2) is well known as the negative regulator of p53. MDM2 has been reported to be overexpressed in SLE animal model and to promote SLE. Since abnormally expressed proteins can induce autoimmune response, anti-MDM2 autoantibody was examined in SLE patients. Methods. Anti-MDM2 antibody in sera from 43 SLE patients and 69 healthy persons was investigated by ELISA. Positive samples were further confirmed by western blotting. The immunological feathers of anti-MDM2 positive sera were analyzed by indirect immunofluorescence assay. Anti-p53 was also investigated in SLE patients by ELISA, and the correlation of anti-MDM2 and anti-p53 was analyzed. Results. The presence of anti-MDM2 in SLE patients was 23.30%, much higher than normal healthy persons (4.30%). These anti-MDM2 positive sera present a nuclear staining pattern. The presence of anti-p53 in SLE patients was 39.50%, and the titer of anti-MDM2 was positively correlated with anti-p53 in SLE patients. Conclusions. Anti-MDM2 autoantibody was detected at high prevalence in SLE patients. The detection of anti-MDM2 in SLE patients should be clinically useful.

摘要

简介。系统性红斑狼疮 (SLE) 是一种以多克隆自身抗体产生为特征的自身免疫性疾病。人源双微体 2 (MDM2) 是 p53 的负调控因子，在 SLE 动物模型中被报道过表达，并促进 SLE。由于异常表达的蛋白质可诱导自身免疫反应，因此在 SLE 患者中检测抗 MDM2 自身抗体。方法。通过 ELISA 检测 43 例 SLE 患者和 69 例健康对照者血清中的抗 MDM2 抗体。阳性样本通过 Western blot 进一步确认。通过间接免疫荧光分析检测抗 MDM2 阳性血清的免疫特征。通过 ELISA 检测 SLE 患者的抗 p53，分析抗 MDM2 和抗 p53 的相关性。结果。SLE 患者抗 MDM2 的阳性率为 23.30%，明显高于正常健康人（4.30%）。这些抗 MDM2 阳性血清呈现核染色模式。SLE 患者抗 p53 的阳性率为 39.50%，SLE 患者抗 MDM2 的滴度与抗 p53 呈正相关。结论。在 SLE 患者中检测到高频率的抗 MDM2 自身抗体。在 SLE 患者中检测抗 MDM2 可能具有临床意义。