Laboureur Laurent, Ollero Mario, Touboul David
CNRS, Institut de Chimie des Substances Naturelles, UPR2301, Université Paris-Sud, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
Institut Mondor de Recherche Biomédicale, INSERM, U955 (eq. 21), Hôpital Henri Mondor, 51 Avenue du Maréchal de Lattre de Tassigny, 94010 Créteil, France.
Int J Mol Sci. 2015 Jun 17;16(6):13868-84. doi: 10.3390/ijms160613868.
This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the injection of extracts in pure organic solvent, this technique is perfectly suitable for lipid analysis and can be coupled with either highly universal (UV or evaporative light scattering) or highly specific (mass spectrometry) detection methods. A short history of the use of supercritical fluids as mobile phase for the separation oflipids will be introduced first. Then, the advantages and drawbacks of SFC are discussed for each class of lipids (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, polyketides) defined by the LIPID MAPS consortium.
本综述阐述了超临界流体色谱法（SFC）在脂质分析领域的作用。SFC在20世纪80年代末和90年代很流行，后来由于液相色谱（LC）的商业成功而几乎消失。直到20年后，当新的商业仪器推出时，人们才重新对其产生兴趣。由于SFC与在纯有机溶剂中进样提取物完全兼容，该技术非常适合脂质分析，并且可以与高度通用的（紫外或蒸发光散射）或高度特异的（质谱）检测方法联用。首先将介绍超临界流体作为流动相用于脂质分离的简短历史。然后，针对脂质代谢组学协会定义的每一类脂质（脂肪酰基、甘油脂质、甘油磷脂、鞘脂、甾醇、异戊二烯醇、聚酮化合物），讨论SFC的优缺点。
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