环状ITCH通过调控Wnt/β-连环蛋白信号通路在结直肠癌中发挥抑制作用。
cir-ITCH plays an inhibitory role in colorectal cancer by regulating the Wnt/β-catenin pathway.
作者信息
Huang Guanli, Zhu Hua, Shi Yixiong, Wu Wenzhi, Cai Huajie, Chen Xiangjian
机构信息
Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
Departments of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
出版信息
PLoS One. 2015 Jun 25;10(6):e0131225. doi: 10.1371/journal.pone.0131225. eCollection 2015.
Noncoding RNAs (ncRNAs) are the dominant product of eukaryotic transcription. These products range from short microRNAs (miRNAs) to long intergenic noncoding RNAs (lincRNAs). Circular RNAs composed of exonic sequences represent an understudied form of ncRNA that was discovered more than 20 years ago. Using a TaqMan-based reverse transcriptase polymerase chain reaction assay, we analyzed the relationship between cir-ITCH expression and colorectal cancer (CRC) in a total of 45 CRCs and paired adjacent non-tumor tissue samples. We found that cir-ITCH expression was typically down-regulated in CRC compared to the peritumoral tissue. This result, as well as several follow-up experiments, showed that cir-ITCH could increase the level of ITCH, which is involved in the inhibition of the Wnt/β-catenin pathway. Therefore, our results showed that cir-ITCH plays a role in CRC by regulating the Wnt/β-catenin pathway.
非编码RNA（ncRNAs）是真核生物转录的主要产物。这些产物范围从短的微小RNA（miRNAs）到长链基因间非编码RNA（lincRNAs）。由外显子序列组成的环状RNA是一种研究较少的ncRNA形式，它在20多年前就被发现了。我们使用基于TaqMan的逆转录聚合酶链反应分析方法，在总共45例结直肠癌（CRC）及配对的相邻非肿瘤组织样本中分析了cir-ITCH表达与结直肠癌之间的关系。我们发现，与肿瘤周围组织相比，cir-ITCH表达在CRC中通常下调。这一结果以及几项后续实验表明，cir-ITCH可以增加ITCH的水平，而ITCH参与Wnt/β-连环蛋白信号通路的抑制。因此，我们的结果表明cir-ITCH通过调节Wnt/β-连环蛋白信号通路在CRC中发挥作用。
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