Vilibic-Cavlek Tatjana, Pem-Novosel Iva, Kaic Bernard, Babić-Erceg Andrea, Kucinar Jasmina, Klobucar Ana, Medic Alan, Pahor Djana, Barac-Juretic Katija, Gjenero-Margan Ira
Department of Virology, Croatian National Institute of Public Health, School of Medicine University of Zagreb , Zagreb , Croatia.
Acta Microbiol Immunol Hung. 2015 Jun;62(2):199-206. doi: 10.1556/030.62.2015.2.9.
During 2011-2012, a total of 1008 serum samples from randomly selected inhabitants of seven Croatian counties located on the Adriatic Coast were tested for the presence of chikungunya virus (CHIKV) IgG antibodies using indirect immunofluorescence assay. Nine participants (0.9%) from four counties were found to be seropositive to CHIKV. Seroprevalence varied from 0.5% to 1.8% between counties. Additionally, a total of 3,699 mosquitoes were captured in 126 localities from August 16 to September 24, 2011. Three mosquito species were found: Ae. albopictus (3010/81.4%), Cx. pipiens (688/18.6%) and only one specimen of the Cs. longiareolata. Female mosquitoes (N = 1,748) were pooled. All pools tested negative for CHIKV RNA using a real-time RT-PCR.
在2011年至2012年期间,使用间接免疫荧光法对从亚得里亚海沿岸七个克罗地亚县随机选取的居民的1008份血清样本进行了基孔肯雅病毒(CHIKV)IgG抗体检测。发现来自四个县的九名参与者(0.9%)对CHIKV血清呈阳性。各县之间的血清阳性率从0.5%到1.8%不等。此外,在2011年8月16日至9月24日期间,在126个地点共捕获了3699只蚊子。发现了三种蚊子:白纹伊蚊(3010只/81.4%)、致倦库蚊(688只/18.6%),长跗库蚊仅捕获到一只标本。将雌蚊(N = 1748只)合并。使用实时逆转录聚合酶链反应对所有样本池进行CHIKV RNA检测,结果均为阴性。