肿瘤坏死因子α增强全反式维甲酸诱导的早幼粒细胞白血病细胞的凋亡
[Tumor necrosis factor alpha enhances apoptosis of all-trans retinoic acid-induced promyelocytic leukemia cells].
作者信息
Li Menglu, Yang Huan, Wang Changying, Yao Zhi, Gao Ying
机构信息
Tianjin Key Laboratory of Cellular and Molecular Immunology, Ministry of Education Key Laboratory of Immunological Microenvironment and Diseases, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Jul;31(7):869-72, 878.
OBJECTIVE
To study the effect of tumor necrosis factor alpha (TNF-α) on apoptosis of human promyelocytic leukemia cells induced by all-trans retinoic acid (ATRA).
METHODS
Human acute promyelocytic leukemia NB4 cells were treated either by ATRA or by ATRA plus TNF-α. Cell proliferation and apoptosis were detected by cell counting and annexin V-FITC/PI staining, respectively. Changes of CD11b expression on NB4 cells were observed by flow cytometry.
RESULTS
Compared with NB4 cells treated with ATRA alone, NB4 cells treated with ATRA plus TNF-α showed slower proliferation and a higher rate of apoptosis during the whole process of differentiation, and had a higher ratio of CD11b positive cells on the second day of differentiation.
CONCLUSION
TNF-α may have potential for strengthening the differentiation and apoptosis of acute promyelocytic leukemia cells induced by ATRA.
目的
研究肿瘤坏死因子α（TNF-α）对全反式维甲酸（ATRA）诱导的人早幼粒细胞白血病细胞凋亡的影响。
方法
人急性早幼粒细胞白血病NB4细胞分别用ATRA或ATRA加TNF-α处理。分别通过细胞计数和膜联蛋白V-异硫氰酸荧光素/碘化丙啶染色检测细胞增殖和凋亡。通过流式细胞术观察NB4细胞上CD11b表达的变化。
结果
与单独用ATRA处理的NB4细胞相比，用ATRA加TNF-α处理的NB4细胞在整个分化过程中增殖较慢且凋亡率较高，并且在分化第二天CD11b阳性细胞的比例更高。
结论
TNF-α可能具有增强ATRA诱导的急性早幼粒细胞白血病细胞分化和凋亡的潜力。
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