用多孔硅纳米针绘制人食管黏膜中的局部胞质酶活性图谱。
Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles.
作者信息
Chiappini Ciro, Campagnolo Paola, Almeida Carina S, Abbassi-Ghadi Nima, Chow Lesley W, Hanna George B, Stevens Molly M
机构信息
Department of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, SW7 2AZ, London, UK.
Department of Surgery and Cancer, Imperial College London, W2 1PG, London, UK.
出版信息
Adv Mater. 2015 Sep 16;27(35):5147-52. doi: 10.1002/adma.201501304. Epub 2015 Jul 21.
Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide-based Cathepsin B cleavable sensor over a large array of nano-needles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma.
多孔硅纳米针能够绘制组织蛋白酶B在正常和肿瘤人类食管黏膜中的活性图谱。在大量纳米针上组装基于肽的组织蛋白酶B可裂解传感器，能够在混合培养物中区分癌细胞和健康细胞。将同样的传感器应用于组织，可以在食管腺癌的肿瘤边缘区域以高分辨率绘制组织蛋白酶B的活性图谱。
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