[采用高效液相色谱法测定柴胡中的柴胡皂苷]

[Determination of saikosaponins in radix Bupleuri using HPLC].

作者信息

Dong Y Y, Luo S Q

出版信息

Zhongguo Zhong Yao Za Zhi. 1989 Nov;14(11):678-81, 703-4.

PMID:2619895

Abstract

A high performance liquid chromatographic method for the determination of saikosaponin a, c and d in Radix Bupleuri was developed. YMC-pack A-314 ODS column (6 x 300mm) with an eluent consisting of acetone-water (58:42) at a flow rate of 1.2 ml/min was used. The saikosaponins were monitored by a differential refractometer without being converted into the corresponding diene derivatives in advance. Megestrol was used as the internal standard. The recovery rate of saikosaponins was higher than 95% and the coefficient of variation (CV) was 3.08%. The contents of saikosaponins a, c and d of 13 species of Radix Bupleuri collected in different districts of China were evaluated by this method.

摘要

建立了一种高效液相色谱法测定柴胡中柴胡皂苷a、c和d的含量。采用YMC-pack A-314 ODS柱（6×300mm），以丙酮-水（58:42）为流动相，流速为1.2ml/min。柴胡皂苷不经预先转化为相应的二烯衍生物，用示差折光检测器进行监测。以甲地孕酮为内标。柴胡皂苷的回收率高于95%，变异系数（CV）为3.08%。用该方法对采自中国不同地区的13种柴胡中柴胡皂苷a、c和d的含量进行了测定。