原卟啉IX催化过氧化氢生成单线态氧。
Protoporphyrin IX catalyzed hydrogen peroxide to generate singlet oxygen.
作者信息
Zeng Jun, Sun Qiyin, Su Jihui, Han Jihui, Zhang Quanshi, Jin Yuehui
机构信息
Beijing Big Base (Top Grade) Equipment Co., LTD China ; Wuxi Yiren Cancer Hospital China.
Beijing Big Base (Top Grade) Equipment Co., LTD China.
出版信息
Int J Clin Exp Med. 2015 May 15;8(5):6829-34. eCollection 2015.
AIM
To study the role of protoporphyrin IX (pPIX) in mitochondrial metabolism of hydrogen peroxide (H2O2).
METHODS
O2 (-) specific fluorescent markers DMA (9,10-dimerthylanthracence) and SOSG (Singlet Oxygen Sensor Green reagent) were used for measurement of singlet oxygen ((1)O2). Catalyzing conversion of H2O2 into (1)O2 by pPIX was monitored in vitro under varied H2O2 content, temperature, and PH value in the reaction. Ex vivo mitochondrial model was used to analyze effects of ferrochelatase (FECH) and high energy X-rays on this catalytic reaction.
RESULTS
In complete dark, measurable (1)O2 was generated when 1.5 mM of H2O2 was incubated with 24 μM of pPIX H2O2 at 37°C for 3 hours. Mitochondrial yield of H2O2 was 0.11±0.03 nmole/mg/min. Mitochondrial FECH significantly improve the catalytic ability of pPIX converting H2O2 into (1)O2. At presence of high-energy X-ray, incubation of 14.4 μM of pPIX with 0.54 μM of H2O2 also generated (1)O2, during which the fluorescence density of 1.05 μM of DMA decreased by 41.5% (P < 0.05). This conversion was not observed when pPIX was replaced with structurally similar hematoporphyrin.
CONCLUSION
pPIX can catalyze conversion of H2O2 into (1)O2.
目的
研究原卟啉IX（pPIX）在过氧化氢（H2O2）线粒体代谢中的作用。
方法
使用O2（-）特异性荧光标记物DMA（9,10-二甲基蒽）和SOSG（单线态氧传感器绿色试剂）测量单线态氧（（1）O2）。在反应中，在不同的H2O2含量、温度和pH值条件下，体外监测pPIX催化H2O2转化为（1）O2的过程。使用离体线粒体模型分析亚铁螯合酶（FECH）和高能X射线对该催化反应的影响。
结果
在完全黑暗中，当1.5 mM的H2O2与24 μM的pPIX H2O2在37°C孵育3小时时，可检测到（1）O2的生成。H2O2的线粒体产量为0.11±0.03纳摩尔/毫克/分钟。线粒体FECH显著提高了pPIX将H2O2转化为（1）O2的催化能力。在高能X射线存在的情况下，14.4 μM的pPIX与0.54 μM的H2O2孵育也产生了（1）O2，在此期间，1.05 μM的DMA荧光密度下降了41.5%（P<0.05）。当用结构相似的血卟啉取代pPIX时，未观察到这种转化。
结论
pPIX可催化H2O2转化为（1）O2。
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