Nam Nguyen Hoai, Tung Pham The, Ngoc Ninh Thi, Hanh Tran Thi Hong, Thao Nguyen Phuong, Thanh Nguyen Van, Cuong Nguyen Xuan, Thao Do Thi, Huong Tran Thu, Thung Do Cong, Kiem Phan Van, Kim Young Ho, Minh Chau Van
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST).
Chem Pharm Bull (Tokyo). 2015;63(8):636-40. doi: 10.1248/cpb.c15-00273.
Ten biscembranoids (1-10), including the two new compounds sarcophytolides M and N (1 and 2), were isolated from the methanol extract of the Vietnamese soft coral Sarcophyton pauciplicatum. Their structures were elucidated by spectroscopic methods including one dimensional (1D)- and 2D-NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and circular dichroism (CD). The in vitro cytotoxic activity of all isolated compounds against a panel of eight human cancer cell lines including HepG2 (hepatoma cancer), HL-60 (acute leukemia), KB (epidermoid carcinoma), LNCaP (prostate cancer), LU-1 (lung cancer), MCF7 (breast cancer), SK-Mel2 (melanoma), and SW480 (colon adenocarcinoma) was evaluated using the sulforhodamine B colorimetric assay. Among the isolated biscembranoids, 1, 3, 4, 7, 9, and 10 exhibited significant cytotoxic effects and may be selected for further studies of their anticancer activity.
从越南软珊瑚少叉肉芝软珊瑚（Sarcophyton pauciplicatum）的甲醇提取物中分离出10种双柏烷类化合物（1-10），包括两种新化合物肉芝醇M和N（1和2）。通过包括一维（1D）和二维核磁共振（NMR）、高分辨率电喷雾电离质谱（HR-ESI-MS）和圆二色性（CD）在内的光谱方法对其结构进行了阐明。使用磺酰罗丹明B比色法评估了所有分离化合物对包括HepG2（肝癌）、HL-60（急性白血病）、KB（表皮样癌）、LNCaP（前列腺癌）、LU-1（肺癌）、MCF7（乳腺癌）、SK-Mel2（黑色素瘤）和SW480（结肠腺癌）在内的八种人类癌细胞系面板的体外细胞毒性活性。在分离出的双柏烷类化合物中，1、3、4、7、9和10表现出显著的细胞毒性作用，可能被选作进一步研究其抗癌活性之用。
