Raducheva T, Velianov D, Ilieva L
Acta Microbiol Bulg. 1989;24:29-34.
The kinetics of growth in vitro of strains Yersinia enterocolitica, serovars 0:3, 0:5, 0:8 and 0:9, on culture media containing rhamnose, raffinose and sodium succinate and at different temperatures of cultivation (4 degrees, 25 degrees and 37 degrees C) was characterized. It was established, that all the Yersinia enterocolitica serovars tested, cultivated at 25 degrees C, had the highest growth rate. A significant activation of growth at this temperature was observed when L-rhamnose and especially sodium succinate were used as substrates. The cultivation at 37 degrees C caused a sharp increase in the growth rate which, however, decreased at the end of the experiment (96th hour of cultivation), and certain stimulation of growth was observed on D-raffinose-containing medium. The growth of cells cultivated at 4 degrees C using L-rhamnose as substrate started after a lag-phase of 24 hours, which was better expressed for serovars 0:8 and 0:9. The results reported could be applied to experiments, using methods of isolation of Yersinia enterocolitica from different sources after cultivation at 4 degrees C on L-rhamnose-containing media.
对小肠结肠炎耶尔森氏菌0:3、0:5、0:8和0:9血清型菌株在含有鼠李糖、棉子糖和琥珀酸钠的培养基上,以及在不同培养温度(4℃、25℃和37℃)下的体外生长动力学进行了表征。结果表明,所有测试的小肠结肠炎耶尔森氏菌血清型在25℃培养时生长速率最高。当使用L-鼠李糖尤其是琥珀酸钠作为底物时,在该温度下观察到生长显著激活。在37℃培养导致生长速率急剧增加,然而在实验结束时(培养96小时)生长速率下降,并且在含D-棉子糖的培养基上观察到一定的生长刺激。以L-鼠李糖为底物在4℃培养的细胞生长在24小时的滞后期后开始,这在0:8和0:9血清型中表现得更明显。所报道的结果可应用于在含L-鼠李糖的培养基上于4℃培养后从不同来源分离小肠结肠炎耶尔森氏菌的实验方法。