125I粒子持续低剂量率辐射与60Co高剂量率γ辐射对非小细胞肺癌细胞生物学效应的比较
A comparison of the biological effects of 125I seeds continuous low-dose-rate radiation and 60Co high-dose-rate gamma radiation on non-small cell lung cancer cells.
作者信息
Wang Zhongmin, Zhao Zhenzhen, Lu Jian, Chen Zhijin, Mao Aiwu, Teng Gaojun, Liu Fenju
机构信息
Institution of Molecular Imaging, Southeast University, Nanjing, China; Department of Interventional Radiology, The Third Affiliated Hospital of the Medical College Shihezi University, Xinjiang, China; Department of Radiology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Radiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Interventional Radiology, Shanghai St. Luke's Hospital, Shanghai, China.
出版信息
PLoS One. 2015 Aug 12;10(8):e0133728. doi: 10.1371/journal.pone.0133728. eCollection 2015.
OBJECTIVES
To compare the biological effects of 125I seeds continuous low-dose-rate (CLDR) radiation and 60Co γ-ray high-dose-rate (HDR) radiation on non-small cell lung cancer (NSCLC) cells.
MATERIALS AND METHODS
A549, H1299 and BEAS-2B cells were exposed to 125I seeds CLDR radiation or 60Co γ-ray HDR radiation. The survival fraction was determined using a colony-forming assay. The cell cycle progression and apoptosis were detected by flow cytometry (FCM). The expression of the apoptosis-related proteins caspase-3, cleaved-caspase-3, PARP, cleaved-PARP, BAX and Bcl-2 were detected by western blot assay.
RESULTS
After irradiation with 125I seeds CLDR radiation, there was a lower survival fraction, more pronounced cell cycle arrest (G1 arrest and G2/M arrest in A549 and H1299 cells, respectively) and a higher apoptotic ratio for A549 and H1299 cells than after 60Co γ-ray HDR radiation. Moreover, western blot assays revealed that 125I seeds CLDR radiation remarkably up-regulated the expression of Bax, cleaved-caspase-3 and cleaved-PARP proteins and down-regulated the expression of Bcl-2 proteins in A549 and H1299 cells compared with 60Co γ-ray HDR radiation. However, there was little change in the apoptotic ratio and expression of apoptosis-related proteins in normal BEAS-2B cells receiving the same treatment.
CONCLUSIONS
125I seeds CLDR radiation led to remarkable growth inhibition of A549 and H1299 cells compared with 60Co HDR γ-ray radiation; A549 cells were the most sensitive to radiation, followed by H1299 cells. In contrast, normal BEAS-2B cells were relatively radio-resistant. The imbalance of the Bcl-2/Bax ratio and the activation of caspase-3 and PARP proteins might play a key role in the anti-proliferative effects induced by 125I seeds CLDR radiation, although other possibilities have not been excluded and will be investigated in future studies.
目的
比较¹²⁵I粒子连续低剂量率（CLDR）辐射与⁶⁰Coγ射线高剂量率（HDR）辐射对非小细胞肺癌（NSCLC）细胞的生物学效应。
材料与方法
将A549、H1299和BEAS-2B细胞暴露于¹²⁵I粒子CLDR辐射或⁶⁰Coγ射线HDR辐射。采用集落形成试验测定存活分数。通过流式细胞术（FCM）检测细胞周期进程和凋亡情况。采用蛋白质免疫印迹法检测凋亡相关蛋白caspase-3、裂解的caspase-3、PARP、裂解的PARP、BAX和Bcl-2的表达。
结果
¹²⁵I粒子CLDR辐射后，与⁶⁰Coγ射线HDR辐射相比，A549和H1299细胞的存活分数更低，细胞周期阻滞更明显（A549和H1299细胞分别为G1期阻滞和G2/M期阻滞），凋亡率更高。此外，蛋白质免疫印迹分析显示与⁶⁰Coγ射线HDR辐射相比，¹²⁵I粒子CLDR辐射显著上调了A549和H1299细胞中Bax、裂解的caspase-3和裂解的PARP蛋白的表达，下调了Bcl-2蛋白的表达。然而，接受相同处理的正常BEAS-2B细胞的凋亡率和凋亡相关蛋白的表达几乎没有变化。
结论
与⁶⁰Co HDRγ射线辐射相比，¹²⁵I粒子CLDR辐射对A549和H1299细胞具有显著的生长抑制作用；A549细胞对辐射最敏感，其次是H1299细胞。相比之下，正常BEAS-2B细胞相对抗辐射。Bcl-2/Bax比值的失衡以及caspase-3和PARP蛋白 的激活可能在¹²⁵I粒子CLDR辐射诱导的抗增殖效应中起关键作用，尽管其他可能性尚未排除，将在未来研究中进行调查。
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