[软性接触镜佩戴者角膜上皮变化的透射电子显微镜观察]

[Corneal epithelial changes of soft contact lens wearers under a transmission electron microscope].

作者信息

Gu Hao, Tang Li

出版信息

Zhonghua Yan Ke Za Zhi. 2015 Mar;51(3):183-7.

Abstract

OBJECTIVE

To investigate the difference in corneal epithelium between patients with and without soft contact lens (SCL) wearing, and to analyze corneal epithelial changes of the eyes with long-time SCL wearing.

METHODS

In this cross sectional study, the subjects were divided into two groups: 13 patients of daily SCL wearers and 11 control subjects who had never worn contact lenses. The flap of corneal epithelium was observed by transmission electron microscopy. The corneal epithelial microvillus density was compared between the two groups.

RESULTS

Transmission electron micrographs of the control group showed a tight connection between cells, regularly aligned basal cells, and continuous basement membrane. Compared with the control group, SCL wearers showed incomplete basement membrane, swollen epithelial cells, swollen mitochondria, and widened intercellular interstices. The density of corneal epithelium microvilli [(0.071466 +/- 0.015889)/microm2 vs. (0.139851 +/- 0.024171)/micro2] was lower (t = 8.312, P < 0.05).

CONCLUSION

Long-term SCL wearing can induce remarkable changes of corneal epithelial tissue, and the density of corneal epithelial microvilli decreases.

摘要

目的

研究佩戴与未佩戴软性接触镜（SCL）患者的角膜上皮差异，并分析长期佩戴SCL患者眼睛的角膜上皮变化。

方法

在这项横断面研究中，受试者被分为两组：13例每日佩戴SCL的患者和11例从未佩戴过隐形眼镜的对照受试者。通过透射电子显微镜观察角膜上皮瓣。比较两组之间的角膜上皮微绒毛密度。

结果

对照组的透射电子显微镜图像显示细胞间紧密连接、基底细胞排列规则以及基底膜连续。与对照组相比，佩戴SCL的患者显示基底膜不完整、上皮细胞肿胀、线粒体肿胀以及细胞间隙增宽。角膜上皮微绒毛密度[（0.071466±0.015889）/μm² 对（0.139851±0.024171）/μm²]较低（t = 8.312，P < 0.05）。