Vidal-Naquet A, Gossage J L, Sullivan T P, Haynes J W, Gilruth B H, Beissinger R L, Sehgal L R, Rosen A L
Department of Chemical Engineering, Illinois Institute of Technology, Chicago.
Biomater Artif Cells Artif Organs. 1989;17(5):531-52. doi: 10.3109/10731198909117635.
Encapsulation methods using high pressure extrusion and homogenization were developed which produce hemoglobin-containing liposomes, approximately one micron or less in diameter with an oxygen-carrying capacity of more than half that of red blood cells (RBCs). These methods were developed for scaling-up liposome-encapsulated hemoglobin (LEH) production. Previously, the lack of adequate scale-up methods has been a serious barrier to full scale efficacy and toxicity testing for all the researchers engaged in such investigations. The scale-up methods and characterization of the resulting LEH preparations are presented.
开发了使用高压挤压和均质化的封装方法，该方法可生产含血红蛋白的脂质体，其直径约为一微米或更小，携氧能力超过红细胞（RBC）的一半。这些方法是为扩大脂质体包裹血红蛋白（LEH）的生产而开发的。以前，缺乏适当的扩大规模方法一直是所有从事此类研究的研究人员进行全面疗效和毒性测试的严重障碍。本文介绍了扩大规模的方法以及所得LEH制剂的特性。
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