埃塞俄比亚中南部古拉格和西尔蒂地区传统医学所用植物的体外抗菌活性
In vitro antimicrobial activity of plants used in traditional medicine in Gurage and Silti Zones, south central Ethiopia.
作者信息
Teka Alemtshay, Rondevaldova Johana, Asfaw Zemede, Demissew Sebsebe, Van Damme Patrick, Kokoska Ladislav, Vanhove Wouter
机构信息
Laboratory for Tropical and Subtropical Agriculture and Ethnobotany, Department of Plant Production, Faculty of Bio-Science Engineering, Ghent University, Coupure links, 653-9000, Ghent, Belgium.
Department of Plant Biology and Biodiversity Management, College of Natural Sciences, Addis Ababa University, P.O. Box 3434, Addis Ababa, Ethiopia.
出版信息
BMC Complement Altern Med. 2015 Aug 18;15:286. doi: 10.1186/s12906-015-0822-1.
BACKGROUND
To overcome the escalating problems associated with infectious diseases and drug resistance, discovery of new antimicrobials is crucial. The present study aimed to carry out in vitro antimicrobial analysis of 15 medicinal plant species selected according to their traditional medicinal uses in Gurage and Silti Zones, south central Ethiopia.
METHODS
Ethanol extracts of various plant parts were investigated for their antimicrobial activity against 20 bacterial and one yeast strains. The minimum inhibitory concentration (MIC) was determined by broth microdilution method.
RESULTS
Asparagus africanus, Guizotia schimperi, Lippia adoensis var. adoensis and Premna schimperi were active against Candida albicans, Enterococcus faecalis and Staphylococcus aureus at a concentration of 512 μg/ml or lower. Strong antibacterial activity (MIC≥128 μg/ml) was observed for G. schimperi extract against 17 resistant and sensitive Staphylococcus strains, at a concentration comparable to standard antibiotics. Moreover, this extract showed higher antibacterial activity for the test against S. aureus ATCC 33591, ATCC 33592, SA3 and SA5 strains (128-256 μg/ml) than oxacillin (512 μg/ml).
CONCLUSIONS
The study revealed in vitro antibacterial activity of plants used in folk medicine in south central Ethiopia. The usefulness of these plants, in particular of G. schimperi, should be confirmed through further phytochemical and toxicity analyses.
背景
为克服与传染病和耐药性相关的不断升级的问题，发现新型抗菌药物至关重要。本研究旨在对根据埃塞俄比亚中南部古拉格和西尔蒂地区传统药用用途挑选的15种药用植物进行体外抗菌分析。
方法
研究了各种植物部位的乙醇提取物对20种细菌菌株和1种酵母菌株的抗菌活性。通过肉汤微量稀释法测定最低抑菌浓度（MIC）。
结果
非洲天门冬、施氏小葵子、阿多利叶下珠和施氏豆腐柴在浓度为512μg/ml或更低时对白色念珠菌、粪肠球菌和金黄色葡萄球菌具有活性。施氏小葵子提取物对17种耐药和敏感葡萄球菌菌株表现出较强的抗菌活性（MIC≥128μg/ml），其浓度与标准抗生素相当。此外，该提取物对金黄色葡萄球菌ATCC 33591、ATCC 33592、SA3和SA5菌株的测试显示出比苯唑西林（512μg/ml）更高的抗菌活性（128 - 256μg/ml）。
结论
该研究揭示了埃塞俄比亚中南部民间医学中使用的植物的体外抗菌活性。这些植物，特别是施氏小葵子的有效性，应通过进一步的植物化学和毒性分析来证实。
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