控制新绿球藻培养过程中的原生动物污染和脂质积累：pH 和溶解无机碳的影响。
Control of protozoa contamination and lipid accumulation in Neochloris oleoabundans culture: Effects of pH and dissolved inorganic carbon.
机构信息
Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
Department of Chemical and Biological Engineering, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
出版信息
Bioresour Technol. 2015 Dec;197:143-51. doi: 10.1016/j.biortech.2015.07.101. Epub 2015 Aug 1.
Combined effects of pH (i.e., 7.5, 8.5, and 9.5) and bicarbonate (i.e., 0, 80 and 160mM NaHCO3) on lipid accumulation and on biological contaminant viability in a protozoa-contaminated culture of the freshwater microalga Neochloris oleoabundans were studied. Cultures grown in the media containing 160mM NaHCO3 at pH 9.5 obtained the highest biomass concentration (DCWmax=1.32g/L), lipid content (LC=327mg/g), which corresponded to a lipid productivity of 56mg/(L·d), and the culture was protozoa free one day after inoculation. Other cultures, 160mM NaHCO3 at pH 8.5 (DCWmax=1.32g/L, LC=223mg/g), and 80mM NaHCO3 at pH 9.5 (DCWmax=1.25g/L, LC=264mg/g) could delay protozoan growth, but not inhibit it completely. These results suggest 160mM NaHCO3 or slightly above at pH levels of 8.5-9.5 may be used in outdoor cultivation processes of freshwater N. oleoabundans to control protozoa contamination while maintain a high lipid content.
研究了 pH 值（分别为 7.5、8.5 和 9.5）和碳酸氢盐（分别为 0、80 和 160mM NaHCO3）对淡水微藻 Neochloris oleoabundans 污染培养物中脂质积累和生物污染物生存能力的综合影响。在 pH 值为 9.5 且含有 160mM NaHCO3 的培养基中生长的培养物获得了最高的生物量浓度（DCWmax=1.32g/L）、脂质含量（LC=327mg/g），对应的脂质生产力为 56mg/（L·d），并且在接种后一天培养物中没有原生动物。其他培养物，如在 pH 值为 8.5 且含有 160mM NaHCO3（DCWmax=1.32g/L，LC=223mg/g）和在 pH 值为 9.5 且含有 80mM NaHCO3（DCWmax=1.25g/L，LC=264mg/g）的条件下，可以延迟原生动物的生长，但不能完全抑制。这些结果表明，在 8.5-9.5 的 pH 值下，使用 160mM NaHCO3 或略高于该浓度，可能用于淡水 N. oleoabundans 的户外培养过程中，在控制原生动物污染的同时保持高脂质含量。
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