Hernández-Flores L, Llanderal-Cázares C, Guzmán-Franco A W, Aranda-Ocampo S
Colegio de Postgraduados. Campus Montecillo. Km 36.5 Carretera México-Texcoco. Montecillo, Estado de México, México.
J Med Entomol. 2015 Sep;52(5):1150-8. doi: 10.1093/jme/tjv099. Epub 2015 Jul 16.
The external and internal culturable bacterial community present in the larvae of Comadia redtenbacheri Hammerschmidt, an edible insect, was studied. Characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16S rRNA gene revealed the existence of Paenibacillus sp., Bacillus safensis, Pseudomonas sp., Bacillus pseudomycoides, Corynebacterium variabile, Enterococcus sp., Gordonia sp., Acinetobacter calcoaceticus, Arthrobacter sp., Micrococcus sp., and Bacillus cereus. Greater diversity of bacteria was found in those larvae obtained from vendors than in those directly taken from Agave plants in nature. Many of the larvae obtained from vendors presented signs of potential disease, and after the analysis, results showed a greater bacterial community compared with the larvae with a healthy appearance. This indicates that bacterial flora can vary in accordance with how the larvae are handled during extraction, collection, and transport.
对可食用昆虫红纹透翅蛾(Comadia redtenbacheri Hammerschmidt)幼虫体内外可培养细菌群落进行了研究。对分离菌株的表征确定了18种形态型的存在,16S rRNA基因的系统发育分析揭示了芽孢杆菌属(Paenibacillus sp.)、安全芽孢杆菌(Bacillus safensis)、假单胞菌属(Pseudomonas sp.)、假霉菌芽孢杆菌(Bacillus pseudomycoides)、可变棒状杆菌(Corynebacterium variabile)、肠球菌属(Enterococcus sp.)、戈登氏菌属(Gordonia sp.)、醋酸钙不动杆菌(Acinetobacter calcoaceticus)、节杆菌属(Arthrobacter sp.)、微球菌属(Micrococcus sp.)和蜡样芽孢杆菌(Bacillus cereus)的存在。与直接从自然界龙舌兰植物中获取的幼虫相比,从供应商处获得的幼虫中发现的细菌多样性更高。从供应商处获得的许多幼虫呈现出潜在疾病的迹象,分析后结果显示,与外观健康的幼虫相比,其细菌群落更多。这表明细菌菌群会根据幼虫在提取、收集和运输过程中的处理方式而有所不同。