微小 RNA-1 能有效地诱导鼠骨髓间充质干细胞向心肌细胞分化。

MicroRNA-1 effectively induces differentiation of myocardial cells from mouse bone marrow mesenchymal stem cells.

机构信息

a Department of Cardiology , Renmin Hospital of Wuhan University , Wuhan , Hubei , China and.

b Department of Geriatrics , Xuan Wu Hospital, Capital Medical University , Beijing , China.

出版信息

Artif Cells Nanomed Biotechnol. 2016 Nov;44(7):1665-70. doi: 10.3109/21691401.2015.1080168. Epub 2015 Sep 16.

DOI:10.3109/21691401.2015.1080168

PMID:26376009

Abstract

In this research, bone marrow mesenchymal stem cells (BMSCs) were isolated from mouse, and induced differentiation into myocardial cells in vitro after overexpression of miR-1a. The results showed that the BMSCs could induce differentiation into myocardial cells under the special condition medium, but when the miR-1a was over-expressed in BMSCs, the differentiation efficiency and induction time of myocardial cells from BMSCs could be promoted. This reason was demonstrated that Delta-like 1 (Dll-1) was a transcriptional repressor of myocardium gene expression during myocardium differentiation, miR-1a reduced Dll-1 levels, leading to the accumulation of myocardium gene mRNA and a dramatic increase in myocardium gene protein.

摘要

在这项研究中，从老鼠中分离出骨髓间充质干细胞（BMSCs），并在 miR-1a 过表达后在体外诱导分化为心肌细胞。结果表明，BMSCs 在特殊条件培养基下可诱导分化为心肌细胞，但当 BMSCs 中过表达 miR-1a 时，可促进 BMSCs 分化为心肌细胞的效率和诱导时间。其原因表明，Delta-like 1（Dll-1）在心肌分化过程中是心肌基因表达的转录抑制因子，miR-1a 降低了 Dll-1 的水平，导致心肌基因 mRNA 的积累和心肌基因蛋白的显著增加。