点、线、面状细胞构建体用于 3D 组织组装。
Point-, line-, and plane-shaped cellular constructs for 3D tissue assembly.
机构信息
Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST), Komaba Open Laboratory (KOL) Room M202, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Takeuchi Biohybrid Innovation Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology (JST), Komaba Open Laboratory (KOL) Room M202, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
出版信息
Adv Drug Deliv Rev. 2015 Dec 1;95:29-39. doi: 10.1016/j.addr.2015.09.003. Epub 2015 Sep 18.
Microsized cellular constructs such as cellular aggregates and cell-laden hydrogel blocks are attractive cellular building blocks to reconstruct 3D macroscopic tissues with spatially ordered cells in bottom-up tissue engineering. In this regard, microfluidic techniques are remarkable methods to form microsized cellular constructs with high production rate and control of their shapes such as point, line, and plane. The fundamental shapes of the cellular constructs allow for the fabrication of larger arbitrary-shaped tissues by assembling them. This review introduces microfluidic formation methods of microsized cellular constructs and manipulation techniques to assemble them with control of their arrangements. Additionally, we show applications of the cellular constructs to biological studies and clinical treatments and discuss future trends as their potential applications.
微细胞构建体，如细胞聚集体和细胞填充水凝胶块，是在自下而上的组织工程中重建具有空间有序细胞的 3D 宏观组织的有吸引力的细胞构建块。在这方面，微流控技术是形成具有高生产率和形状控制（如点、线和平面）的微细胞构建体的出色方法。细胞构建体的基本形状允许通过组装它们来制造更大的任意形状的组织。本综述介绍了微细胞构建体的微流控形成方法和操纵技术，以控制其排列方式对其进行组装。此外，我们展示了细胞构建体在生物研究和临床治疗中的应用，并讨论了它们作为潜在应用的未来趋势。
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