阔叶榆绿木和银背藤提取物在动物模型中的镇痛和抗炎活性评价
Evaluation of Analgesic and Anti-Inflammatory Activity of Extract of Holoptelea Integrifolia and Argyreia Speciosa in Animal Models.
作者信息
Lalan Bhavesh K, Hiray R S, Ghongane B B
机构信息
Assistant Manager-Medical Services, Unichem Laboratories Ltd ., Mumbai, India .
Associate Professor, Department of Pharmacology, B. J. Govt Medical College and Sassoon General Hospitals , Pune, India .
出版信息
J Clin Diagn Res. 2015 Jul;9(7):FF01-4. doi: 10.7860/JCDR/2015/12059.6200. Epub 2015 Jul 1.
BACKGROUND
Long term use of NSAIDs, opioids and corticosteroids was associated with serious adverse effects. Hence, the search for a safer analgesic and anti-inflammatory agent was always going on. It was considered worthwhile to evaluate analgesic and anti-inflammatory activities of Holoptelea integrifolia and Argyreia speciosa.
AIM
To evaluate analgesic and anti-inflammatory activities of aqueous extract of leaves of Holoptelea Integrifolia and methanolic extract of Argyreia Speciosa root powder in mice and rats.
MATERIALS AND METHODS
After obtaining permission from animal ethics committee, the animals were divided into 7 groups of 6 animals each {control, standard - ibuprofen 100mg/kg, Holoptelea integrifolia (250 and 500 mg/kg), Argyreia speciosa (100 and 300 mg/kg) and combination of Holoptelea integrifolia (250 mg/kg) and Argyreia speciosa (100 mg/kg)}. The analgesic activity of the extracts was evaluated using tail-flick with radiant heat and acetic acid induced writhing method and the anti-inflammatory activity was evaluated using carrageenan induced paw oedema method.
STATISTICAL ANALYSIS
One-way ANOVA followed by post-hoc test. p < 0.05 was considered to be significant.
RESULTS
In tail-flick method, both Holoptelea integrifolia and Argyreia speciosa produced significant (p<0.05) increase in latency as compared to control, their combination showed a significant increase in latency as compared to control as well as to the standard - ibuprofen. In writhing method, Holoptelea integrifolia and Argyreia speciosa, alone and in combination, significantly decreased the number of writhes as compared to control. In paw oedema method, both Holoptelea integrifolia and Argyreia speciosa showed significant inhibition of paw oedema as compared to control and the activity was comparable to ibuprofen.
CONCLUSION
Extracts of Holoptelea integrifolia and Argyreia speciosa exhibits significant central and peripheral analgesic and anti-inflammatory activity.
背景
长期使用非甾体抗炎药、阿片类药物和皮质类固醇与严重不良反应相关。因此，一直在寻找更安全的镇痛和抗炎药物。评估全缘叶朴和美丽银背藤的镇痛和抗炎活性被认为是有价值的。
目的
评估全缘叶朴叶水提取物和美丽银背藤根粉甲醇提取物对小鼠和大鼠的镇痛和抗炎活性。
材料与方法
获得动物伦理委员会许可后，将动物分为7组，每组6只动物{对照组、标准组 - 布洛芬100mg/kg、全缘叶朴（250和500mg/kg）、美丽银背藤（100和300mg/kg）以及全缘叶朴（250mg/kg）和美丽银背藤（100mg/kg）的组合}。采用辐射热甩尾法和醋酸诱导扭体法评估提取物的镇痛活性，采用角叉菜胶诱导足爪肿胀法评估抗炎活性。
统计分析
采用单因素方差分析及事后检验。p < 0.05被认为具有显著性。
结果
在甩尾法中，与对照组相比，全缘叶朴和美丽银背藤均使潜伏期显著延长（p<0.05），它们的组合与对照组以及标准组布洛芬相比，潜伏期也显著延长。在扭体法中，与对照组相比，全缘叶朴和美丽银背藤单独及联合使用均显著减少了扭体次数。在足爪肿胀法中，与对照组相比，全缘叶朴和美丽银背藤均显著抑制了足爪肿胀，且活性与布洛芬相当。
结论
全缘叶朴和美丽银背藤提取物具有显著的中枢和外周镇痛及抗炎活性。
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