Sudarshan Medhavi, Singh Toolika, Chakravarty Jaya, Sundar Shyam
J Clin Microbiol. 2015 Dec;53(12):3905-7. doi: 10.1128/JCM.01465-15.
Parasitological diagnosis of visceral leishmaniasis (VL) by splenic smear is highly sensitive, but it is associated with the risk of severe hemorrhage. In this study, the diagnosis of VL using quantitative PCR (qPCR) in peripheral blood was evaluated in 100 patients with VL. Blood parasitemia ranged from 5 to 93,688 leishmania parasite genomes/ml of blood and positively correlated with splenic score (P<0.0001; r2=0.58). Therefore, quantification of parasite genomes by qPCR can replace invasive procedures for diagnostic and prognostic evaluations.
通过脾脏涂片对内脏利什曼病(VL)进行寄生虫学诊断具有高度敏感性,但它与严重出血风险相关。在本研究中,对100例VL患者进行了利用外周血定量PCR(qPCR)诊断VL的评估。血中寄生虫血症范围为每毫升血液5至93,688个利什曼原虫基因组,且与脾脏评分呈正相关(P<0.0001;r2=0.58)。因此,通过qPCR对寄生虫基因组进行定量可替代侵入性操作用于诊断和预后评估。