Yuan Xiao-Long, Cao Min, Bi Gui-Qi
a College of Marine Life Sciences, Ocean University of China , Qingdao , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2777-8. doi: 10.3109/19401736.2015.1053061. Epub 2015 Sep 24.
Pseudo-nitzschia multiseries belongs to Baciuariophyta, which can produce the neurotoxin known as domoic acid, a toxin that can cause amnesic shellfish poisoning in human beings. The research aspects mainly focus on its toxicity and diversity. However, there is no mitochondrial genome information about P. multiseries. The complete mitochondrial genome of P. multiseries was obtained through amplifying. The mitogenome is 46 283 bp in length with 31.04% GC content. It has a typical cyclic structure. The genome contains 37 protein-coding genes, including unidentified open reading frames, 23 tRNAs and 2rRNAs. Four group II introns were found in rps5, cob, cox1 and cox2. The co-linear analysis result exhibited existence of gene modules re-arrangements and inversions among P. multiseries, Synedra acus and Thalassiosira pseudonana. The gene contents comparison showed they have high similarities. Phylogeny results suggest P. multiseries has a close evolutionary relationship with T. pseudonana.
多列拟菱形藻属于硅藻门,能够产生一种名为软骨藻酸的神经毒素,这种毒素会导致人类失忆性贝类中毒。研究方面主要集中在其毒性和多样性上。然而,目前尚无关于多列拟菱形藻的线粒体基因组信息。通过扩增获得了多列拟菱形藻的完整线粒体基因组。该线粒体基因组长度为46283bp,GC含量为31.04%。它具有典型的环状结构。该基因组包含37个蛋白质编码基因,包括未鉴定的开放阅读框、23个tRNA和2个rRNA。在rps5、cob、cox1和cox2中发现了4个II类内含子。共线性分析结果表明,多列拟菱形藻、针杆藻和假微型海链藻之间存在基因模块重排和倒位现象。基因含量比较显示它们具有高度相似性。系统发育结果表明,多列拟菱形藻与假微型海链藻具有密切的进化关系。