Dong Xiaolong, Li Qiujia, Zhang Hongyu
State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Dev Comp Immunol. 2016 Feb;55:233-40. doi: 10.1016/j.dci.2015.09.009. Epub 2015 Sep 21.
The noa gene is an essential gene encoding a very long chain fatty acid elongase. In this study, we cloned the noa gene of Bactrocera dorsalis, which encodes a protein sharing 84.50% identity to the NOA in Drosophila melanogaster. The expression profiles indicated that the transcriptional level of noa was high at the egg stage and in the testis tissue. The results showed that noa expression was up-regulated after Listeria monocytogenes, Staphylococcus aureus and Escherichia coli infection. Silencing of noa would influence the expression of immune related genes, including MyD88 and defensin in the Toll pathway and relish and diptericin in the Imd pathway. Moreover, infection with L. monocytogenes and S. aureus after feeding ds-noa, the expression of MyD88 and defensin down-regulated significantly in ds-noa group compared with in ds-egfp group, indicating that noa interference influenced the activation of the Toll pathway. Meanwhile, infection with L. monocytogenes and E. coli, which activated the Imd pathway, do not cause increase of the mRNA levels of relish and diptericin in ds-noa group as severely as in ds-egfp treatment, indicating that the Imd pathway was also repressed after silences of noa.
noa基因是一个编码超长链脂肪酸延长酶的必需基因。在本研究中，我们克隆了桔小实蝇的noa基因，该基因编码的蛋白质与黑腹果蝇中的NOA具有84.50%的同一性。表达谱表明，noa在卵期和睾丸组织中的转录水平较高。结果显示，单核细胞增生李斯特菌、金黄色葡萄球菌和大肠杆菌感染后noa表达上调。noa基因沉默会影响免疫相关基因的表达，包括Toll途径中的MyD88和防御素，以及Imd途径中的relish和双翅肽。此外，喂食ds-noa后感染单核细胞增生李斯特菌和金黄色葡萄球菌，与ds-egfp组相比，ds-noa组中MyD88和防御素的表达显著下调，表明noa干扰影响了Toll途径的激活。同时，感染激活Imd途径的单核细胞增生李斯特菌和大肠杆菌后，ds-noa组中relish和双翅肽的mRNA水平没有ds-egfp处理组那样严重升高，表明noa沉默后Imd途径也受到抑制。
