Lv Ying-Ying, Wang Jia, You Jia, Qiu Li-Hong
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, PR China.
Int J Syst Evol Microbiol. 2015 Dec;65(12):4816-4822. doi: 10.1099/ijsem.0.000653. Epub 2015 Oct 5.
A Gram-reaction-negative, aerobic, non-motile bacterial strain, DHOC24T, was isolated from the forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Strain DHOC24T underwent a shape change during the course of culture from long filamentous cells (10-30 × 0.4-0.5 μm) at 2 days to coccobacilli (0.5-1.0 × 0.7-1.0 μm) at 15 days after inoculation. It grew optimally at 28-33 °C and pH 6.5-7.5. The major quinone of strainDHOC24T was MK-7, the main fatty acids were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH and the DNA G+C content was 43.1 mol%. On the basis of 16S rRNA gene sequence analysis, the strain was found to be affiliated with members of the genus Chitinophaga, but was clearly separated from established species of the genus. Strain DHOC24T was most closely related to Chitinophaga jiangningensis JN53T (98.3 % 16S rRNA gene sequence similarity) and Chitinophaga terrae KP01T (97.9 %). DNA-DNA hybridization study showed relatively low relatedness values (32.1 %) of strain DHOC24T with C. jiangningensis JN53T. The phenotypic, chemotaxonomic and phylogenetic data showed that strain DHOC24T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga dinghuensis sp. nov. is proposed. The type strain is DHOC24T ( = CGMCC 1.12995T = DSM 29821T).
从中国广东省鼎湖山生物圈保护区的森林土壤中分离出一株革兰氏阴性、需氧、不运动的细菌菌株DHOC24T。菌株DHOC24T在培养过程中形态发生变化，接种后2天时长丝状细胞（10 - 30×0.4 - 0.5μm），15天时变为球杆菌（0.5 - 1.0×0.7 - 1.0μm）。其最适生长温度为28 - 33℃，最适pH为6.5 - 7.5。菌株DHOC24T的主要醌为MK - 7，主要脂肪酸为异C15∶0、C16∶1ω5c和异C17∶0 3 - OH，DNA G + C含量为43.1mol%。基于16S rRNA基因序列分析，该菌株属于噬几丁质菌属成员，但与该属已确立的物种明显分开。菌株DHOC24T与江宁噬几丁质菌JN53T（16S rRNA基因序列相似性为98.3%）和土壤噬几丁质菌KP01T（97.9%）关系最为密切。DNA - DNA杂交研究表明，菌株DHOC24T与江宁噬几丁质菌JN53T的相关性相对较低（32.1%）。表型、化学分类和系统发育数据表明，菌株DHOC24T代表噬几丁质菌属的一个新物种，为此提出新名称鼎湖噬几丁质菌（Chitinophaga dinghuensis sp. nov.）。模式菌株为DHOC24T（= CGMCC 1.12995T = DSM 29821T）。
