检测口咽鳞状细胞癌（OPSCC）患者和非口咽鳞状细胞癌患者口腔冲洗液样本中的人乳头瘤病毒（HPV）。
Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients.
作者信息
Dang Juliet, Feng Qinghua, Eaton Keith D, Jang Hona, Kiviat Nancy B
机构信息
Oral Health Sciences, University of Washington, Box 357475, 1959 NE Pacific St., Seattle, WA, 98195, USA.
University of Washington, 1959 NE Pacific St., Seattle, WA, 98195, USA.
出版信息
BMC Oral Health. 2015 Oct 15;15(1):126. doi: 10.1186/s12903-015-0111-x.
BACKGROUND
Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples.
METHODS
In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients.
RESULTS
Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18).
CONCLUSION
Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.
背景
由于口咽癌发病率不断上升，口腔人乳头瘤病毒（HPV）感染成为一个重大问题。由于存在不同的检测技术，检测口腔HPV的方法尚未标准化。我们认为，在临床环境中，使用漱口水样本检测HPV是一种合适的技术。因此，我们的主要目标是研究漱口水样本中的HPV检测情况。
方法
在我们的研究中，我们采用漱口水样本采集结合实时聚合酶链反应（PCR）来检测口咽鳞状细胞癌（OPSCC）、非OPSCC和健康患者中的16型和18型HPV，并优先扩增FAP PCR样本以检测多种HPV。
结果
100名癌症患者中有33%的人任何类型的HPV检测呈阳性；其中23人HPV16检测呈阳性。110名健康对照者中只有1人呈阳性（该受试者HPV18检测呈阳性）。
结论
我们的结果表明，漱口水样本中的HPV检测可能作为一种筛查工具，用于检测与HPV相关的口腔癌。
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