通过核糖体谱分析在基因组尺度上进行翻译分析。

Translation Analysis at the Genome Scale by Ribosome Profiling.

作者信息

Baudin-Baillieu Agnès, Hatin Isabelle, Legendre Rachel, Namy Olivier

机构信息

Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA, CNRS, Université Paris Sud, Bâtiment 400, 91405, Orsay, France.

出版信息

Methods Mol Biol. 2016;1361:105-24. doi: 10.1007/978-1-4939-3079-1_7.

DOI:10.1007/978-1-4939-3079-1_7

PMID:26483019

Abstract

Ribosome profiling is an emerging approach using deep sequencing of the mRNA part protected by the ribosome to study protein synthesis at the genome scale. This approach provides new insights into gene regulation at the translational level. In this review we describe the protocol to prepare polysomes and extract ribosome protected fragments before to deep sequence them.

摘要

核糖体谱分析是一种新兴的方法，通过对核糖体保护的mRNA部分进行深度测序，在基因组规模上研究蛋白质合成。这种方法为翻译水平的基因调控提供了新的见解。在这篇综述中，我们描述了制备多聚核糖体和提取核糖体保护片段以便进行深度测序之前的实验方案。