Ohba Seigo, Sumita Yoshinori, Umebayashi Mayumi, Yoshimura Hitoshi, Yoshida Hisato, Matsuda Shinpei, Kimura Hideki, Asahina Izumi, Sano Kazuo
Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan; Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Arch Oral Biol. 2016 Jan;61:23-7. doi: 10.1016/j.archoralbio.2015.10.012. Epub 2015 Oct 22.
The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone. The mice were sacrificed and the specimens were harvested four weeks after surgery. The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.
The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline. Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline. However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.
The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
本研究旨在使用一种新型人工骨材料——羟基磷灰石/胶原蛋白复合材料，结合全血或富血小板血浆，评估小鼠颅骨上新形成的覆盖骨。
将含有生理盐水、全血或富血小板血浆的羟基磷灰石/胶原蛋白复合材料移植到小鼠颅骨上。术后四周处死小鼠并采集标本。使用Image J软件在苏木精和伊红染色的标本上测量新形成的骨面积。
与含有生理盐水的复合材料相比，含有全血或富血小板血浆的羟基磷灰石/胶原蛋白复合材料诱导形成的新骨量显著更多。此外，术后四周在含有全血或富血小板血浆的移植材料中观察到了骨髓，而含有生理盐水的材料中未观察到。然而，使用全血与富血小板血浆的材料之间新形成的骨量没有显著差异。
羟基磷灰石/胶原蛋白复合材料对于覆盖骨增大有效，并且该材料在移植前应浸泡在全血或富血小板血浆中。
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