酵母固定化细胞流化床生物反应器系统在乳糖水解乳清和乳清渗透液乙醇发酵中的动力学
Dynamics of yeast immobilized-cell fluidized-bed bioreactors systems in ethanol fermentation from lactose-hydrolyzed whey and whey permeate.
作者信息
Gabardo Sabrina, Pereira Gabriela Feix, Klein Manuela P, Rech Rosane, Hertz Plinho F, Ayub Marco Antônio Záchia
机构信息
Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
Enzymology Laboratory, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
出版信息
Bioprocess Biosyst Eng. 2016 Jan;39(1):141-50. doi: 10.1007/s00449-015-1498-0. Epub 2015 Nov 2.
We studied the dynamics of ethanol production on lactose-hydrolyzed whey (LHW) and lactose-hydrolyzed whey permeate (LHWP) in batch fluidized-bed bioreactors using single and co-cultures of immobilized cells of industrial strains of Saccharomyces cerevisiae and non-industrial strains of Kluyveromyces marxianus. Although the co-culture of S. cerevisiae CAT-1 and K. marxianus CCT 4086 produced two- to fourfold the ethanol productivity of single cultures of S. cerevisiae, the single cultures of the K. marxianus CCT 4086 produced the best results in both media (Y EtOH/S = 0.47-0.49 g g(-1) and Q P = 1.39-1.68 g L(-1) h(-1), in LHW and LHWP, respectively). Ethanol production on concentrated LHWP (180 g L(-1)) reached 79.1 g L(-1), with yields of 0.46 g g(-1) for K. marxianus CCT 4086 cultures. Repeated batches of fluidized-bed bioreactor on concentrated LHWP led to increased ethanol productivity, reaching 2.8 g L(-1) h(-1).
我们使用酿酒酵母工业菌株和马克斯克鲁维酵母非工业菌株的固定化细胞单培养物和共培养物，在分批流化床生物反应器中研究了乳糖水解乳清（LHW）和乳糖水解乳清渗透液（LHWP）上乙醇的生产动力学。尽管酿酒酵母CAT-1和马克斯克鲁维酵母CCT 4086的共培养物产生的乙醇生产率是酿酒酵母单培养物的两到四倍，但马克斯克鲁维酵母CCT 4086的单培养物在两种培养基中均产生了最佳结果（在LHW和LHWP中，Y EtOH/S分别为0.47 - 0.49 g g(-1)和QP为1.39 - 1.68 g L(-1) h(-1)）。在浓缩LHWP（180 g L(-1)）上的乙醇产量达到79.1 g L(-1)，马克斯克鲁维酵母CCT 4086培养物的产率为0.46 g g(-1)。在浓缩LHWP上重复进行分批流化床生物反应器操作可提高乙醇生产率，达到2.8 g L(-1) h(-1)。
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