C57BL/6小鼠中系统性红斑狼疮（SLE）的bm12诱导模型。
The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice.
作者信息
Klarquist Jared, Janssen Edith M
机构信息
Division of Immunobiology, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine.
Division of Immunobiology, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine;
出版信息
J Vis Exp. 2015 Nov 1(105):e53319. doi: 10.3791/53319.
Systemic lupus erythematosus (SLE) is an autoimmune disease with diverse clinical and immunological manifestations. Several spontaneous and inducible animal models mirror common components of human disease, including the bm12 transfer model. Upon transfer of bm12 splenocytes or purified CD4 T cells, C57BL/6 mice rapidly develop large frequencies of T follicular helper cells (Tfh), germinal center (GC) B cells, and plasma cells followed by high levels of circulating anti-nuclear antibodies. Since this model utilizes mice on a pure C57BL/6 background, researchers can quickly and easily study disease progression in transgenic or knockout mouse strains in a relatively short period of time. Here we describe protocols for the induction of the model and the quantitation Tfh, GC B cells, and plasma cells by multi-color flow cytometry. Importantly, these protocols can also be used to characterize disease in most mouse models of SLE and identify Tfh, GC B cells, and plasma cells in other disease models.
系统性红斑狼疮（SLE）是一种具有多种临床和免疫学表现的自身免疫性疾病。几种自发和诱导性动物模型反映了人类疾病的常见组成部分，包括bm12转移模型。在转移bm12脾细胞或纯化的CD4 T细胞后，C57BL/6小鼠迅速产生大量频率的滤泡辅助性T细胞（Tfh）、生发中心（GC）B细胞和浆细胞，随后出现高水平的循环抗核抗体。由于该模型使用的是纯C57BL/6背景的小鼠，研究人员可以在相对较短的时间内快速轻松地研究转基因或基因敲除小鼠品系中的疾病进展。在这里，我们描述了该模型的诱导方案以及通过多色流式细胞术对Tfh、GC B细胞和浆细胞进行定量的方法。重要的是，这些方案也可用于表征大多数SLE小鼠模型中的疾病，并在其他疾病模型中鉴定Tfh、GC B细胞和浆细胞。
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