酿酒酵母线粒体中存在DNA引发酶的证据。
Evidence for the presence of DNA primase in mitochondria of Saccharomyces cerevisiae.
作者信息
Desai S D, Pasupathy K, Chetty K G, Pradhan D S
机构信息
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
出版信息
Biochem Biophys Res Commun. 1989 Apr 28;160(2):525-34. doi: 10.1016/0006-291x(89)92464-9.
Chromatography of a DNA polymerase preparation from mitochondria of Saccharomyces cerevisiae on DNA-cellulose column, using Tris-HCl (pH 7.5) buffer containing 0.6 M NaCl as eluent, was found to yield a fraction exhibiting DNA primase-like activity free of DNA polymerase. This fraction could support the synthesis of 12-15 residue-long oligoribonucleotides on single-stranded natural or synthetic DNA templates. The oligoribonucleotides could be further elongated by incorporation of deoxyribonucleotides in the presence of Klenow fragment.
用含0.6M NaCl的Tris-HCl（pH 7.5）缓冲液作为洗脱剂，对来自酿酒酵母线粒体的DNA聚合酶制剂进行DNA-纤维素柱层析，结果发现得到了一个不含DNA聚合酶但具有DNA引发酶样活性的组分。该组分能够在单链天然或合成DNA模板上支持合成12 - 15个残基长的寡核糖核苷酸。在Klenow片段存在的情况下，通过掺入脱氧核糖核苷酸，这些寡核糖核苷酸可以进一步延长。
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