5-羟甲基胞嘧啶和5-甲酰基胞嘧啶在哺乳动物的DNA和RNA中的存在。
The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals.
作者信息
Zhang Hao-Ying, Xiong Jun, Qi Bao-Ling, Feng Yu-Qi, Yuan Bi-Feng
机构信息
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China.
出版信息
Chem Commun (Camb). 2016 Jan 14;52(4):737-40. doi: 10.1039/c5cc07354e.
We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.
我们开发了一种通过氧化衍生化结合质谱分析的新策略，用于测定DNA和RNA中的5-羟甲基胞嘧啶和5-甲酰基胞嘧啶。我们报道了哺乳动物RNA中5-甲酰基胞嘧啶的存在，并发现抗坏血酸和对苯二酚可以增加DNA和RNA中5-甲基胞嘧啶向5-羟甲基胞嘧啶的氧化。
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