从塔比木蝴蝶树皮中分离出的凝集素/抑制剂（CrataBL）对控制黄斑豆象幼虫发育的潜力。
Potential of the Lectin/Inhibitor Isolated from Crataeva tapia Bark (CrataBL) for Controlling Callosobruchus maculatus Larva Development.
作者信息
Nunes Natalia N S, Ferreira Rodrigo S, Silva-Lucca Rosemeire A, de Sá Leonardo F R, de Oliveira Antônia Elenir A, Correia Maria Tereza dos S, Paiva Patrícia Maria G, Wlodawer Alexander, Oliva Maria Luiza V
机构信息
Departamento de Bioquı́mica, Universidade Federal de São Paulo-UNIFESP-EPM , 04044-020 São Paulo, SP, Brazil.
Centro de Engenharia e Ciências Exatas, Universidade Estadual do Oeste do Paraná , Toledo, Paraná, Brazil.
出版信息
J Agric Food Chem. 2015 Dec 9;63(48):10431-6. doi: 10.1021/acs.jafc.5b03634. Epub 2015 Dec 1.
Callosobruchus maculatus is an important predator of cowpeas. Due to infestation during storage, this insect affects the quality of seed and crop yield. This study aimed to investigate the effects of CrataBL, a multifunction protein isolated from Crataeva tapia bark, on C. maculatus larva development. The protein, which is stable even in extreme pH conditions, showed toxic activity, reducing the larval mass 45 and 70% at concentrations of 0.25 and 1.0% (w/w), respectively. Acting as an inhibitor, CrataBL decreased by 39% the activity of cysteine proteinases from larval gut. Conversely, the activity of serine proteinases was increased about 8-fold. The toxic properties of CrataBL may also be attributed to its capacity of binding to glycoproteins or glycosaminoglycans. Such binding interferes with larval metabolism, because CrataBL-FITC was found in the fat body, Malpighian tubules, and feces of larvae. These results demonstrate the potential of this protein for controlling larva development.
绿豆象是豇豆的一种重要害虫。由于在储存期间受到侵害，这种昆虫会影响种子质量和作物产量。本研究旨在探讨从塔比木蝴蝶树皮中分离出的多功能蛋白CrataBL对绿豆象幼虫发育的影响。该蛋白即使在极端pH条件下也很稳定，具有毒性活性，在浓度为0.25%和1.0%（w/w）时，分别使幼虫体重降低了45%和70%。作为一种抑制剂，CrataBL使幼虫肠道中半胱氨酸蛋白酶的活性降低了39%。相反，丝氨酸蛋白酶的活性增加了约8倍。CrataBL的毒性特性也可能归因于其与糖蛋白或糖胺聚糖结合的能力。这种结合会干扰幼虫的新陈代谢，因为在幼虫的脂肪体、马氏管和粪便中发现了CrataBL-FITC。这些结果证明了这种蛋白在控制幼虫发育方面的潜力。
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