猝灭剂、变性剂、温度及pH值对牛血清白蛋白保护的金纳米簇荧光性质的影响
Effect of Quencher, Denaturants, Temperature and pH on the Fluorescent Properties of BSA Protected Gold Nanoclusters.
作者信息
Chib Rahul, Butler Susan, Raut Sangram, Shah Sunil, Borejdo Julian, Gryczynski Zygmunt, Gryczynski Ignacy
机构信息
Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA.
Department of Cell Biology and Immunology, Center for Fluorescence Technologies and Nanomedicine, University of North Texas Health Science Center, Fort Worth, TX, 76107,USA ; Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX, 76129, USA.
出版信息
J Lumin. 2015 Dec 1;168:62-68. doi: 10.1016/j.jlumin.2015.07.030.
In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The phtophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment. Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties.
在本文中，我们合成了牛血清白蛋白保护的金纳米簇（BSA金纳米簇），并研究了猝灭剂、蛋白质变性剂、pH值和温度对BSA金纳米簇及天然牛血清白蛋白中色氨酸分子荧光性质的影响。我们还研究了它们对BSA金纳米簇（650nm）发射峰的影响。在不同环境中对新开发的荧光团进行光物理表征对于进一步开发其生物医学和分析应用绝对必要。从我们的实验中观察到，BSA金纳米簇中的色氨酸能更好地免受极性环境的影响。天然牛血清白蛋白中的色氨酸在其发射峰波长位置出现红移。色氨酸是一种对极性高度敏感的染料，其微环境的微小变化很容易在其光物理性质中观察到。
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