凝胶基免疫分析法的建立及其在猪肉中沙丁胺醇和莱克多巴胺残留快速筛选中的应用
Development and Application of a Gel-Based Immunoassay for the Rapid Screening of Salbutamol and Ractopamine Residues in Pork.
机构信息
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , 100193 Beijing, People's Republic of China.
The Engineering Laboratory of Synthetic Biology, Key Laboratory of Biomedical Engineering, School of Medicine, Health Science Center, Shenzhen University , 518060 Shenzhen, People's Republic of China.
出版信息
J Agric Food Chem. 2015 Dec 9;63(48):10556-61. doi: 10.1021/acs.jafc.5b04203. Epub 2015 Dec 1.
Salbutamol (SAL) and ractopamine (RAC) have been illegally used to promote protein synthesis and to increase the feed conversion rate in livestock. However, the residues of SAL and RAC could cause potential hazards for human health. The Ministry of Agriculture of China banned the use of SAL and RAC as growth promoters. In this paper, we provide detailed information on developing a rapid and sensitive gel-based immunoassay for on-site screening of SAL and RAC residues in pork. The detection time was shortened to 20 min. The limits of detection were 0.5 μg/kg for both SAL and RAC by visual detection, whereas the quantitative gel-based immunoassay enabled the detection of SAL (0.051 μg/kg) and RAC (0.020 μg/kg) in spiked pork samples. The gel-based immunoassay showed promise as a multiplexed immunoassay for on-site surveilling of SAL and RAC residues in pork.
沙丁胺醇（SAL）和莱克多巴胺（RAC）已被非法用于促进蛋白质合成和提高牲畜的饲料转化率。然而，SAL 和 RAC 的残留会对人类健康造成潜在危害。中国农业部已禁止将 SAL 和 RAC 用作生长促进剂。在本文中，我们提供了详细信息，介绍了如何快速灵敏地开发一种基于凝胶的免疫测定法，用于现场筛选猪肉中的 SAL 和 RAC 残留。检测时间缩短至 20 分钟。通过目视检测，SAL 和 RAC 的检出限均为 0.5μg/kg，而基于凝胶的定量免疫测定法可检测到添加猪肉样品中的 SAL（0.051μg/kg）和 RAC（0.020μg/kg）。该基于凝胶的免疫测定法有望成为一种用于现场监测猪肉中 SAL 和 RAC 残留的多重免疫测定法。
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