微小RNA-374a通过靶向轴抑制蛋白2促进骨肉瘤细胞增殖。
MiR-374a promotes the proliferation of osteosarcoma cell proliferation by targeting Axin2.
作者信息
Lu Tan, Zhang Chao, Chai Ming-Xiang, An Yong-Bo, Jia Jin-Ling
机构信息
Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
出版信息
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10776-83. eCollection 2015.
BACKGROUND
MicroRNA-374a (miR-374a) has been implicated in several cancers. However, its role in osteosarcoma (OS) remains unclear. Thus the aim of this study was to investigate its expression and role in progression of OS.
METHODS
Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of miR-374a in OS cell lines and tissues. To further understand its role, we restored expression of miR-374a in MG63 cell line by transfection with miR-374a mimics or inhibitors. Effects of miR-374a on cell proliferation on targets were also determined.
RESULTS
In the present study, our results showed that miR-374a was significantly up-regulated in both OS cell lines and OS tissues. Over expression of miR-374a markedly accelerated proliferation of OS cells, while its inhibition significantly suppressed cell proliferation. Moreover, Axin2 was identified to be a functional downstream target of miR-374a, and decreased expression of Axin2 could promote OS cell proliferation.
CONCLUSION
Our study suggested that miR-374a functions as an oncogene in OS, and the miR-374a/Axin2 axis might represent a potential therapeutic target for OS intervention.
背景
微小RNA-374a（miR-374a）已被证实与多种癌症有关。然而，其在骨肉瘤（OS）中的作用仍不清楚。因此，本研究旨在探讨其在骨肉瘤进展中的表达及作用。
方法
采用定量实时聚合酶链反应（qRT-PCR）检测miR-374a在骨肉瘤细胞系和组织中的表达。为进一步了解其作用，我们通过转染miR-374a模拟物或抑制剂来恢复MG63细胞系中miR-374a的表达。还确定了miR-374a对细胞增殖靶点的影响。
结果
在本研究中，我们的结果表明miR-374a在骨肉瘤细胞系和骨肉瘤组织中均显著上调。miR-374a的过表达显著加速了骨肉瘤细胞的增殖，而其抑制则显著抑制了细胞增殖。此外，Axin2被确定为miR-374a的功能性下游靶点，Axin2表达的降低可促进骨肉瘤细胞增殖。
结论
我们的研究表明，miR-374a在骨肉瘤中起癌基因作用，miR-374a/Axin2轴可能是骨肉瘤干预的潜在治疗靶点。
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