[502例急性髓系白血病（AML）中cMPO的表达及其在AML亚型中的诊断意义]

[Expression of cMPO in 502 cases of acute myeloid leukemia (AML) and its diagnosis significance in AML subtypes].

作者信息

Shang Lei, Chen Xuejing, Li Yuanyuan, Guo Guiqing, He Dashui, Cai Xiaojin, Zheng Bin, Mi Yingchang, Wang Jianxiang, Ru Kun, Wang Huijun

机构信息

Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2015 Nov;36(11):906-11. doi: 10.3760/cma.j.issn.0253-2727.2015.11.004.

DOI:10.3760/cma.j.issn.0253-2727.2015.11.004

PMID:26632461

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7342422/

Abstract

OBJECTIVE

To investigate the myeloperoxidase (cMPO) expression pattern by flow cytometry (FCM) in patients with acute myeloid leukemia (AML) and its role in classifying AML.

METHODS

Eight- color multiparametric FCM with CD45/SSC gating was used to determine the cMPO expression in 502 AML patients.

RESULTS

The positive rate of cMPO in all patients was 58.0%, in which the proportion of normal positivity, dim positivity and partial positivity was 21.5%, 34.1% and 2.4%, respectively. The remaining case (42.0%) were all negative. In AML with t (15;17）(q22;q12)/PMLRARα, the positive rate was the highest (100%) and the intensity was similar to that of the normal granular leukocytes, followed by AML with t (8;21（q22;q22/RUNX1-RUNX1T1, the positive rate was 91.4% and the intensity was mostly dim. AML with minimal differentiation and acute megakaryoblastic leukemia were all cMPO negative. The positive rates of cMPO in the remaining subtypes were between 22.7% and 76.2%.

CONCLUSION

The positive rate and intensity of cMPO were significantly different among different subtypes of AML.

摘要

目的

采用流式细胞术（FCM）研究急性髓系白血病（AML）患者髓过氧化物酶（cMPO）的表达模式及其在AML分型中的作用。

方法

采用八色多参数FCM结合CD45/SSC设门法检测502例AML患者的cMPO表达。

结果

所有患者中cMPO阳性率为58.0%，其中正常阳性、弱阳性和部分阳性的比例分别为21.5%、34.1%和2.4%。其余病例（42.0%）均为阴性。在伴有t（15；17）（q22；q12）/PML-RARα的AML中，阳性率最高（100%），强度与正常粒细胞相似，其次是伴有t（8；21）（q22；q22）/RUNX1-RUNX1T1的AML，阳性率为91.4%，强度大多为弱阳性。微小分化型AML和急性巨核细胞白血病cMPO均为阴性。其余亚型的cMPO阳性率在22.7%至76.2%之间。